Evaluation of a porcine whole-limb heterotopic autotransplantation model
Article first published online: 13 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 33, Issue 2, pages 141–147, February 2013
How to Cite
Kiermeir, D. M., Meoli, M., Müller, S., Abderhalden, S., Vögelin, E. and Constantinescu, M. A. (2013), Evaluation of a porcine whole-limb heterotopic autotransplantation model. Microsurgery, 33: 141–147. doi: 10.1002/micr.22038
- Issue published online: 7 FEB 2013
- Article first published online: 13 SEP 2012
- Manuscript Accepted: 13 JUL 2012
- Manuscript Revised: 12 JUL 2012
- Manuscript Received: 15 JAN 2012
- Swiss National Science Foundation. Grant Numbers: 3200B0-116618, 32003B-138434
- Swiss National Accident Insurance (SUVA-Schweizerische Unfallversicherungsanstalt)
Recently performed vascularized composite tissue allotransplantations (CTAs) stimulate the ongoing research in the area of whole-limb transplantation. A reliable in vivo animal model is required for investigations in vascularized whole-limb CTA. The model should allow in vivo assessment in whole-limb preservation, allograft and xenograft response, and host immunomodulation. The goal of this study is to describe and evaluate the in vivo feasibility and reproducibility of a whole-limb porcine model as a basis for future research in this field.
Materials and methods:
In seven large white pigs, one forelimb was amputated under anesthesia and autotransplanted heterotopically with an arc of rotation of 180° and partially placed in a subcutaneous pocket. Clinical parameters were monitored and muscle biopsies were analyzed using ultrastructural morphological assessment of mitochondria quality after an observation period of 7 days.
All animals could fully mobilize postoperatively without restrictions. At sacrifice, the anastomosed pedicle vessels of the limb were patent in six animals. In one pig, venous thrombosis could be observed. Muscle response was triggered following direct electrostimulation in six replanted limbs. The replanted extremities gained 12.97% weight within 7 days postreplantation compared with the amputation baseline values (P = 0.464 while maintaining normal compartment pressures at sacrifice (8.25 ± 5.31 cmH2O, P = 0.60). The ultrastructural evaluation of mitochondria morphology revealed intact mitochondria without signs of ischemia/reperfusion damage.
This porcine model proved feasible, reliable, and reproducible for whole-limb autotransplantation. It presents significant potential in future preclinical research of whole-limb CTA transplantation. © 2012 Wiley Periodicals, Inc., Microsurgery, 2013.