Microvascular changes following four-hour single arteriole occlusion
Version of Record online: 14 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 33, Issue 3, pages 207–215, March 2013
How to Cite
Saltzman, D. J., Kerger, H., Jimenez, J. C., Farzan, D., Wilson, J. M., THOMPSON, J. E. and Intaglietta, M. (2013), Microvascular changes following four-hour single arteriole occlusion. Microsurgery, 33: 207–215. doi: 10.1002/micr.22051
- Issue online: 15 MAR 2013
- Version of Record online: 14 NOV 2012
- Manuscript Accepted: 21 AUG 2012
- Manuscript Revised: 13 AUG 2012
- Manuscript Received: 11 FEB 2012
Free tissue transplantations are lengthy procedures that result in prolong tissue ischemia. Restoral of blood flow is essential for free flap recovery; however, upon reperfusion tissue that is viable may continue to be nonperfused. To further elucidate this pathophysiology skeletal muscle microcirculation was investigated during reperfusion following 4-hour single arteriole occlusion.
Materials and methods:
A blunt micropipette probe was use to compress a single arteriole in the unanesthetized hamster (N = 20) dorsal skinfold chamber. Arteriole (n = 20), capillary (n = 97), and postcapillary venule (n = 16) diameters and blood flow were analyzed at 0, 30, 60, 120, 240 min and 24 hours of reperfusion after 4 hour occlusion. Results: Feeding arcade arterioles exhibited a brief (<10 min) vasoconstriction [0.31 ± 0.26 (mean ± SE) of baseline] upon reperfusion followed by a maximum vasodilation at 120 min (1.3 ± 0.10: P < 0.05). Vasodilation was observed in transverse arterioles (A3) (1.8 ± 0.20: P < 0.05). Correspondingly, all arteriole and venule flow was increased by 120 min (P < 0.05) of reperfusion. There was a transient decrease in the number of flowing capillaries at 0 and 30 min reperfusion (0.73 ± 0.09 and 0.84 ± 0.06: P < 0.05, respectively).
At the onset of reperfusion heterogeneous arteriole flow and transient decrease in flowing capillaries was observed; however, return of flow in all capillaries and an eventual hyperemic response in all arterioles suggests the reversible nature of this response. Single arteriole occlusion may allow for a more controlled and detailed microcirculatory analysis during ischemia-reperfusion. © 2012 Wiley Periodicals, Inc. Microsurgery, 2013.