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Expression patterns and functional evaluation of the UNC5B receptor during the early phase of peripheral nerve regeneration using the mouse median nerve model

Authors

  • Patrick Jaminet M.D.,

    Corresponding author
    1. Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Trauma Center, Eberhard Karls University of Tübingen, Tübingen, Germany
    • Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Trauma Center, Eberhard Karl University of Tübingen, Schnarrenbergstr. 95, 72076 Tübingen, Germany
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  • David Köhler Ph.D.,

    1. Department of Anesthesiology and Intensive Care Medicine, University Hospital Tübingen, Eberhard Karls University of Tübingen, Tübingen, Germany
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  • Afshin Rahmanian-Schwarz M.D.,

    1. Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Trauma Center, Eberhard Karls University of Tübingen, Tübingen, Germany
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  • Oliver Lotter M.D.,

    1. Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Trauma Center, Eberhard Karls University of Tübingen, Tübingen, Germany
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  • Alice Mager,

    1. Department of Anesthesiology and Intensive Care Medicine, University Hospital Tübingen, Eberhard Karls University of Tübingen, Tübingen, Germany
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  • Michele Fornaro Ph.D.,

    1. Dipartimento di Scienze Cliniche e Biologiche, Neuroscience Institute Cavalieri Ottolenghi, Università di Torino, Italy
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  • Giulia Ronchi Ph.D.,

    1. Dipartimento di Scienze Cliniche e Biologiche, Neuroscience Institute Cavalieri Ottolenghi, Università di Torino, Italy
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  • Stefano Geuna Ph.D.,

    1. Dipartimento di Scienze Cliniche e Biologiche, Neuroscience Institute Cavalieri Ottolenghi, Università di Torino, Italy
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  • Peter Rosenberger M.D., Ph.D.,

    1. Department of Anesthesiology and Intensive Care Medicine, University Hospital Tübingen, Eberhard Karls University of Tübingen, Tübingen, Germany
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  • Hans-Eberhard Schaller M.D., Ph.D.

    1. Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Trauma Center, Eberhard Karls University of Tübingen, Tübingen, Germany
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Abstract

Introduction: In this study, we evaluated the role of the Netrin-1 receptor UNC5b (Uncoordinated), a neuronal guidance molecule, during peripheral nerve regeneration using the mouse median nerve model. Materials and methods: Using Western blot analysis, we examined the expression changes of UNC5b after transection and microsurgical repair of the mouse median nerve distal to the transection site. We evaluated the histomorphometrical changes and functional recovery of the grasping force after median nerve transection and repair in wild-type (WT) mice and UNC5b+/− heterozygous mice. Results: In Western blot analysis, we could show a high increase of UNC5b in the nerve segment distal to the injury site at day 14. Histomorphometrical analysis did not show any significant differences between WT animals and heterozygous animals. Using the functional grasping test, we could demonstrate that peripheral nerve regeneration is significantly diminished in heterozygous UNC5b+/− mice. Conclusion: By using the mouse median nerve model in transgenic animals, we demonstrate that the Netrin-1 receptor UNC5b plays an important role during peripheral nerve regeneration. © 2012 Wiley Periodicals, Inc. Microsurgery, 2013.

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