Cell lineage in vascularized bone transplantation
Article first published online: 27 AUG 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 34, Issue 1, pages 37–43, January 2014
How to Cite
Willems, W. F., Larsen, M., Friedrich, P. F. and Bishop, A. T. (2014), Cell lineage in vascularized bone transplantation. Microsurgery, 34: 37–43. doi: 10.1002/micr.22147
- Issue published online: 26 DEC 2013
- Article first published online: 27 AUG 2013
- Manuscript Accepted: 17 MAY 2013
- Manuscript Revised: 13 MAY 2013
- Manuscript Received: 20 FEB 2012
- NIH. Grant Number: AR49718
The biology behind vascularized bone allotransplantation remains largely unknown. We aim to study cell traffic between donor and recipient following bone auto-, and allografting.
Vascularized femoral transplantation was performed with arteriovenous bundle implantation and short-term immunosuppression. Twenty male Piebald Virol Glaxo (PVG; RT1c) rats received isotransplants from female PVG (RT1c) rats and 22 male PVG rats received allografts from female Dark Agouti rats (DA, RT1a), representing a major histocompatibility mismatch. Both groups were randomly analyzed at 4 or 18 weeks. Bone remodeling areas (inner and outer cortical samples) were labeled and laser capture microdissected. Analysis of sex-mismatch genes by real-time reverse transcription-polymerase chain reaction provided the relative Expression Ratio (rER) of donor (female) to recipient (male) cells.
The rER was 0.456 ± 0.266 at 4 weeks and 0.749 ± 0.387 at 18 weeks (p = 0.09) in allotransplants. In isotransplants, the rER was 0.412 ± 0.239 and 0.467 ± 0.252 at 4 and 18 weeks, respectively (p = 0.21). At 4 weeks, the rER at the outer cortical area of isotransplants was significantly lower in isotransplants as compared with allotransplants (0.247 ± 0.181 vs. 0.549 ± 0.184, p = 0.007). Cells in the inner and outer cortical bone remodeling areas in isotransplants were mainly donor derived (rER < 0.5) at 18 weeks, whereas allotransplants contained mainly recipient-derived cells (rER > 0.5) at 18 weeks.
Applying novel methodology, we describe detailed cell traffic in vascularized bone transplants, elaborating our comprehension on bone transplantation. © 2013 Wiley Periodicals, Inc. Microsc. Res. Tech. 34:37–43, 2013.