Diosgenin attenuates inflammatory changes in the interaction between adipocytes and macrophages

Authors

  • Shizuka Hirai,

    1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
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    • These authors contributed equally to this work.

  • Taku Uemura,

    1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
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    • These authors contributed equally to this work.

  • Noriko Mizoguchi,

    1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
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  • Joo-Young Lee,

    1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
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  • Keiko Taketani,

    1. Spice Research Laboratory, Somatech Center, House Foods Corporation, Yotsukaido, Chiba, Japan
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  • Yuki Nakano,

    1. Spice Research Laboratory, Somatech Center, House Foods Corporation, Yotsukaido, Chiba, Japan
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  • Shohei Hoshino,

    1. Spice Research Laboratory, Somatech Center, House Foods Corporation, Yotsukaido, Chiba, Japan
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  • Nobuaki Tsuge,

    1. Spice Research Laboratory, Somatech Center, House Foods Corporation, Yotsukaido, Chiba, Japan
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  • Toshihiko Narukami,

    1. Spice Research Laboratory, Somatech Center, House Foods Corporation, Yotsukaido, Chiba, Japan
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  • Rina Yu,

    1. Department of Food Science and Nutrition, University of Ulsan, Ulsan, South Korea
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  • Nobuyuki Takahashi,

    1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
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  • Teruo Kawada

    Corresponding author
    1. Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    • Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan Fax: +81-774-38-3752
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Abstract

Obese adipose tissues are characterized by the enhanced infiltration of macrophages. It is considered that the paracrine loop involving monocyte chemoattractant protein-1, tumor necrosis factor-α, and the free fatty acid between adipocytes and macrophages establishes a vicious cycle that aggravates inflammatory changes and insulin resistance in obese adipose tissues. Diosgenin, a saponin aglycon found in a variety of plants, has anti-inflammatory properties. In the present study, we examined the effect of diosgenin on the inflammatory changes in the interaction between adipocytes and macrophages. A coculture of 3T3-L1 adipocytes and RAW 264 macrophages markedly enhanced the production of tumor necrosis factor-α, monocyte chemoattractant protein-1, and nitric oxide compared with the sum of their single cultures; however, treatment with diosgenin inhibited the production of these proinflammatory mediators. Diosgenin also suppressed the inflammation in RAW 264 macrophages that was induced by the conditioned medium derived from 3T3-L1 adipocytes. Furthermore, diosgenin inhibited the conditioned medium-induced degradation of inhibitor κB and the phosphorylation of c-jun N-terminal kinase in macrophages. These results indicate that diosgenin exhibits anti-inflammatory properties in the interaction of adipocytes and macrophages by inhibiting the inflammatory signals in macrophages. Diosgenin may be useful for ameliorating the inflammatory changes in obese adipose tissues.

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