Colonic availability of polyphenols and D-(−)-quinic acid after apple smoothie consumption
Article first published online: 9 NOV 2010
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 55, Issue 3, pages 368–377, March 2011
How to Cite
Hagl, S., Deusser, H., Soyalan, B., Janzowski, C., Will, F., Dietrich, H., Albert, F. W., Rohner, S. and Richling, E. (2011), Colonic availability of polyphenols and D-(−)-quinic acid after apple smoothie consumption. Mol. Nutr. Food Res., 55: 368–377. doi: 10.1002/mnfr.201000252
- Issue published online: 2 MAR 2011
- Article first published online: 9 NOV 2010
- Manuscript Accepted: 6 SEP 2010
- Manuscript Revised: 31 AUG 2010
- Manuscript Received: 2 JUN 2010
- NutritionNet project supported by the German Federal Ministry of Education and Research. Grant Number: BMBF; 01EA0501
- Rhineland-Palatinate. Grant Number: A4 project
- Apple smoothie;
- Chlorogenic acid;
- D-(−)-Quinic acid;
Scope: The aim of this study was to determine the amounts of polyphenols and D-(−)-quinic acid reaching the ileostomy bags of probands (and thus the colon in healthy humans) after ingestion of apple smoothie, a beverage containing 60% cloudy apple juice and 40% apple puree.
Methods and results: Ten healthy ileostomy subjects each ingested 0.7 L of apple smoothie (a bottle). Their ileostomy bags were collected directly before and 1, 2, 4, 6 and 8 h after smoothie consumption, and the polyphenol and D-(−)-quinic acid contents of the ileostomy fluids were examined using HPLC-DAD and HPLC-MS/MS. The total polyphenol and D-(−)-quinic acid content of the apple smoothie was determined to be 1955.6±124.6 mg/0.7 L, which is very high compared to cloudy apple juices. The most abundant substances found in the ileostomy bags were oligomeric procyanidins (705.6±197.9 mg), D-(−)-quinic acid (363.4±235.5 mg) and 5-caffeoylquinic acid (76.7±26.8 mg). Overall recovery of ingested polyphenols and D-(−)-quinic acid in the ileostomy bags was 63.3±16.1%.
Conclusions: The amounts of polyphenol and D-(−)-quinic acids reaching the ileostomy bags are considerably higher after apple smoothie consumption than after the consumption of cloudy apple juice or cider. These results suggest that the food matrix might affect the colonic availability of polyphenols, and apple smoothies could be more effective in the prevention of chronic colon diseases than both cloudy apple juice and apple cider.