CD36 is involved in lycopene and lutein uptake by adipocytes and adipose tissue cultures

Authors

  • Myriam Moussa,

    1. INRA, UMR1260 “Nutriments Lipidiques et Prévention des Maladies Métaboliques”, Marseille, France
    2. University Aix-Marseille 1, University Aix-Marseille 2, Faculté de Médecine, Marseille, France
    Search for more papers by this author
  • Erwan Gouranton,

    1. INRA, UMR1260 “Nutriments Lipidiques et Prévention des Maladies Métaboliques”, Marseille, France
    2. University Aix-Marseille 1, University Aix-Marseille 2, Faculté de Médecine, Marseille, France
    Search for more papers by this author
  • Béatrice Gleize,

    1. INRA, UMR1260 “Nutriments Lipidiques et Prévention des Maladies Métaboliques”, Marseille, France
    2. University Aix-Marseille 1, University Aix-Marseille 2, Faculté de Médecine, Marseille, France
    3. INSERM, ERL U1025“Bioavailability of micronutrients”, Marseille, France
    Search for more papers by this author
  • Claire El Yazidi,

    1. INRA, UMR1260 “Nutriments Lipidiques et Prévention des Maladies Métaboliques”, Marseille, France
    2. University Aix-Marseille 1, University Aix-Marseille 2, Faculté de Médecine, Marseille, France
    Search for more papers by this author
  • Isabelle Niot,

    1. Physiologie de la Nutrition, UMR INSERM U 866, AgroSup Dijon, Université de Bourgogne, Dijon, France
    Search for more papers by this author
  • Philippe Besnard,

    1. Physiologie de la Nutrition, UMR INSERM U 866, AgroSup Dijon, Université de Bourgogne, Dijon, France
    Search for more papers by this author
  • Patrick Borel,

    1. INRA, UMR1260 “Nutriments Lipidiques et Prévention des Maladies Métaboliques”, Marseille, France
    2. University Aix-Marseille 1, University Aix-Marseille 2, Faculté de Médecine, Marseille, France
    3. INSERM, ERL U1025“Bioavailability of micronutrients”, Marseille, France
    Search for more papers by this author
  • Jean-François Landrier

    Corresponding author
    1. INRA, UMR1260 “Nutriments Lipidiques et Prévention des Maladies Métaboliques”, Marseille, France
    2. University Aix-Marseille 1, University Aix-Marseille 2, Faculté de Médecine, Marseille, France
    • UMR 1260 INRA, Faculté de Médecine, 27 boulevard Jean-Moulin, 13385 Marseille Cedex 5, France Fax: +33-4-91-78-21-01
    Search for more papers by this author

Abstract

Scope: Carotenoids are mainly stored in adipose tissue. However, nothing is known regarding the uptake of carotenoids by adipocytes. Thus, our study explored the mechanism by which lycopene and lutein, two major human plasma carotenoids, are transported.

Methods and results: CD36 was a putative candidate for this uptake, 3T3-L1 cells were treated with sulfosuccinimidyl oleate, a CD36-specific inhibitor. sulfosuccinimidyl oleate-treated cells showed a significant decrease in both lycopene and lutein uptake as compared to control cells. Their uptake was also decreased by partial inhibition of CD36 expression using siRNA, whereas the overexpression of CD36 in Cos-1 cells increased their uptake. Finally, the effect of CD36 on carotenoid uptake was confirmed ex vivo in cultures of adipose tissue explants from CD36−/− mice, which exhibited reduced carotenoid uptake as compared to wild-type mice explants.

Conclusion: For the first time, we report the involvement of a transporter, CD36, in carotenoid uptake by adipocytes and adipose tissue.

Ancillary