Regular endurance exercise improves the diminished hepatic carnitine status in mice fed a high-fat diet
Article first published online: 19 JUL 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Special Issue: Lipids as Effectors
Supplement: Lipids as Effectors
Volume 55, Issue Supplement 2, pages S193–S202, September 2011
How to Cite
Ringseis, R., Mooren, F.-C., Keller, J., Couturier, A., Wen, G., Hirche, F., Stangl, G. I., Eder, K. and Krüger, K. (2011), Regular endurance exercise improves the diminished hepatic carnitine status in mice fed a high-fat diet. Mol. Nutr. Food Res., 55: S193–S202. doi: 10.1002/mnfr.201100040
- Issue published online: 26 SEP 2011
- Article first published online: 19 JUL 2011
- Manuscript Accepted: 17 MAY 2011
- Manuscript Revised: 29 APR 2011
- Manuscript Received: 19 JAN 2011
- Carnitine synthesis;
- Endurance exercise;
- High-fat diet;
- Peroxisome proliferator-activated receptor α
Scope: Metabolic stress induced by chronic high-fat (HF) diet feeding or genetically induced diabetes impairs carnitine status. Herein, we tested the hypothesis that regular endurance exercise (EE) improves the HF diet-induced impairment of carnitine status through stimulating the expression of hepatic genes involved in carnitine synthesis and uptake.
Methods and results: Eighteen male C57BL/6 mice were assigned to three groups: group S received a standard diet, group HF received a HF diet, and group HF+EE received an HF diet and was regularly exercised on a treadmill. After 10 wk, mice of the HF and the HF+EE groups were highly obese and insulin resistant compared with mice of the S group (p<0.05), but mice of the HF+EE group were less insulin resistant than those of the HF group (p<0.05). The HF group had lower carnitine concentrations and mRNA and protein levels of genes involved in carnitine synthesis and uptake in the liver than the S group (p<0.05), whereas these parameters did not differ between the S group and the HF+EE group.
Conclusion: These findings indicate that regular EE reverses an HF diet-induced impairment of hepatic carnitine content by stimulating hepatic carnitine synthesis and uptake.