Dicaffeoylquinic acids in Yerba mate (Ilex paraguariensis St. Hilaire) inhibit NF-κB nucleus translocation in macrophages and induce apoptosis by activating caspases-8 and -3 in human colon cancer cells
Article first published online: 8 JUN 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 55, Issue 10, pages 1509–1522, October 2011
How to Cite
Puangpraphant, S., Berhow, M. A., Vermillion, K., Potts, G. and Gonzalez de Mejia, E. (2011), Dicaffeoylquinic acids in Yerba mate (Ilex paraguariensis St. Hilaire) inhibit NF-κB nucleus translocation in macrophages and induce apoptosis by activating caspases-8 and -3 in human colon cancer cells. Mol. Nutr. Food Res., 55: 1509–1522. doi: 10.1002/mnfr.201100128
- Issue published online: 10 OCT 2011
- Article first published online: 8 JUN 2011
- Manuscript Accepted: 12 APR 2011
- Manuscript Revised: 22 MAR 2011
- Manuscript Received: 26 FEB 2011
- University of Illinois Research Board
- Royal Thai Government Scholarship
- Colon cancer;
- Dicaffeoylquinic acids;
- Yerba mate
Scope: The biological functions of caffeoylquinic acid (CQA) derivatives from various plant sources have been partially elucidated. The objectives were to isolate and purify diCQAs from Yerba mate tea leaves and assess their anti-inflammatory and anti-cancer capabilities in vitro and explore their mechanism of action.
Methods and results: Methanol extracts of dried mate leaves were resolved by flash chromatography and further purified resulting in two fractions one containing 3,4- and 3,5-diCQAs and the other 4,5-diCQA with NMR-confirmed structures. Both fractions inhibited LPS-induced RAW 264.7 macrophage inflammation by suppressing nitric oxide/inducible nitric oxide and prostaglandin E2/cyclooxygenase-2 pathways through inhibiting nucleus translocation of Nuclear factor κB subunits, p50 and p65. The diCQA fractions inhibited Human colon cancer cells CRL-2577 (RKO) and HT-29 cell proliferation by inducing apoptosis in a time- and concentration-dependent manner, but did not affect the protein levels of p21, p27, p53, and Bax:Bcl-2 ratio in RKO cells. In HT-29 cells, however, the diCQA fractions increased Bax:Bcl-2 ratio. The diCQA fractions increased the activation of caspase-8 leading to cleavage of caspase-3 in both RKO and HT-29 colon cancer cells.
Conclusion: The results suggest that diCQAs in Yerba mate could be potential anti-cancer agents and could mitigate other diseases also associated with inflammation.