In vitro anti-platelet effects of simple plant-derived phenolic compounds are only found at high, non-physiological concentrations
Article first published online: 5 SEP 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 55, Issue 11, pages 1624–1636, November 2011
How to Cite
Ostertag, L. M., O'Kennedy, N., Horgan, G. W., Kroon, P. A., Duthie, G. G. and de Roos, B. (2011), In vitro anti-platelet effects of simple plant-derived phenolic compounds are only found at high, non-physiological concentrations. Mol. Nutr. Food Res., 55: 1624–1636. doi: 10.1002/mnfr.201100135
- Issue published online: 2 NOV 2011
- Article first published online: 5 SEP 2011
- Manuscript Accepted: 23 MAY 2011
- Manuscript Revised: 11 MAY 2011
- Manuscript Received: 1 MAR 2011
- Scottish Government Rural and Environment Research and Analysis Directorate (RERAD)
- Biotechnology & Biological Sciences Research Council (BBSRC)
- Cardiovascular disease;
- Dietary phenolics;
- Phenolic acids;
- Platelet aggregation;
- P-selectin expression
Scope: Bioactive polyphenols from fruits, vegetables, and beverages have anti-platelet effects and may thus affect the development of cardiovascular disease. We screened the effects of 26 low molecular weight phenolic compounds on two in vitro measures of human platelet function.
Methods and results: After platelets had been incubated with one of 26 low molecular weight phenolic compounds in vitro, collagen-induced human platelet aggregation and in vitro TRAP-induced P-selectin expression (as marker of platelet activation) were assessed. Incubation of platelet-rich plasma from healthy volunteers with 100 μmol/L hippuric acid, pyrogallol, catechol, or resorcinol significantly inhibited collagen-induced platelet aggregation (all p<0.05; n≥15). Incubation of whole blood with concentrations of 100 μmol/L salicylic acid, p-coumaric acid, caffeic acid, ferulic acid, 4-hydroxyphenylpropionyl glycine, 5-methoxysalicylic acid, and catechol significantly inhibited TRAP-induced surface P-selectin expression (all p<0.05; n=10). Incubation with lower concentrations of phenolics affected neither platelet aggregation nor activation.
Conclusion: As concentrations of 100 μmol/L are unlikely to be reached in the circulation, it is doubtful whether consumption of dietary phenolics in nutritionally attainable amounts plays a major role in inhibition of platelet activation and aggregation in humans.