Metabolites and tissue distribution of resveratrol in the pig
Article first published online: 28 JUN 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Special Issue: Resveratrol – Current Status and Outlook
Volume 55, Issue 8, pages 1154–1168, August 2011
How to Cite
Azorín-Ortuño, M., Yáñez-Gascón, M. J., Vallejo, F., Pallarés, F. J., Larrosa, M., Lucas, R., Morales, J. C., Tomás-Barberán, F. A., García-Conesa, M. T. and Espín, J. C. (2011), Metabolites and tissue distribution of resveratrol in the pig. Mol. Nutr. Food Res., 55: 1154–1168. doi: 10.1002/mnfr.201100140
- Issue published online: 3 AUG 2011
- Article first published online: 28 JUN 2011
- Manuscript Accepted: 18 MAY 2011
- Manuscript Revised: 17 MAY 2011
- Manuscript Received: 2 MAR 2011
- Fundación Seneca de la Region de Murcia (grupo de excelencia GERM 06, 04486). Grant Number: CICYT-BFU2007-60576
- Consolider Ingenio 2010, (Fun-C-Food). Grant Number: CSD2007-00063
Scope: trans-Resveratrol (RES) and/(or) its metabolites exert many effects in vivo. Our aim was to study the metabolism and tissue distribution of RES using the pig, a mammal physiologically close to humans.
Methods and results: Forty-seven tissues, organs and fluids were analyzed 6 h after intragastric RES administration (5.9 mg/kg body weight) using HPLC-MS/MS. Twelve RES and seven dihydroresveratrol (DH-RES) metabolites were detected. DH-RES was the main metabolite in cecum, colon and rectum, whereas RES-3-O-glucuronide was the most abundant one in fluids and organs. Approximately 74.5% of the total RES administered was recovered in the form of RES, DH-RES and derived metabolites (65.1% along the gastrointestinal tract, 7.7% in urine, 1.2% in bile and 0.5% in organs). We report here, for the first time, the occurrence of RES ribosyl-sulfate derivative, DH-RES diglucuronide, DH-RES sulfoglucuronide and DH-RES disulfate as well as the metabolic profile of RES and DH-RES in the aorta, lymph, lymph node, ovaries, uterus, cerebellum, pancreas, urinary bladder tissue, fat and muscle.
Conclusion: This study contributes to the clarification of the metabolism and tissue distribution of RES and could help to further understand the mechanisms underlying its effects.