Soy food supplementation, dietary fat reduction and peripheral blood gene expression in postmenopausal women – A randomized, controlled trial
Article first published online: 8 AUG 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Special Issue: Lipids as Effectors
Supplement: Lipids as Effectors
Volume 55, Issue Supplement 2, pages S264–S277, September 2011
How to Cite
Wang, J., Siegmund, K., Tseng, C.-C., Lee, A. S. and Wu, A. H. (2011), Soy food supplementation, dietary fat reduction and peripheral blood gene expression in postmenopausal women – A randomized, controlled trial. Mol. Nutr. Food Res., 55: S264–S277. doi: 10.1002/mnfr.201100242
- Issue published online: 26 SEP 2011
- Article first published online: 8 AUG 2011
- Manuscript Accepted: 20 JUN 2011
- Manuscript Revised: 3 JUN 2011
- Manuscript Received: 6 APR 2011
- Susan G Komen Breast Cancer Foundation. Grant Numbers: BASIC99-00328, BCTR00-000709
- University of Southern California/Norris Comprehensive Cancer Center Core Grant. Grant Number: 5P03ES007048-13
Vol. 57, Issue 9, 1688, Article first published online: 2 SEP 2013
- Dietary fat reduction;
- Peripheral blood gene expression;
- Soy supplementation
Scope: The effect of soy food supplementation or dietary fat reduction on gene expression is not well studied.
Methods and results: We evaluated the potential of gene expression profiling in peripheral blood mononuclear cells (PBMCs) collected at baseline and at the completion of an 8-wk controlled dietary intervention. Healthy postmenopausal women were randomized to a very-low-fat diet (VLFD; 11% of energy as fat) (n=21), a Step 1 diet (25% energy as fat) supplemented with soy food (SFD; 50 mg isoflavones per day) (n=20), or a control Step 1 diet (CD; 27% energy as fat) with no SFD (n=18). All diets were prepared at the General Clinical Research Center of the University of Southern California. We did not observe any gene that showed variable response across the three dietary interventions. However, there were notable changes in gene expression associated with the intervention in the VLFD and SFD groups. Our findings suggest that the expression of nicotinamide phosphoribosyltransferase (NAMPT) and genes related to Fc γ R-mediated phagocytosis and cytokine interactions may be significantly altered in association with dietary fat reduction and soy supplementation. Gene expression changes in NAMPT were somewhat dampened with adjustment for weight but changes related to Fc γ R-mediated phagocytosis and cytokine interactions remained largely unchanged.
Conclusion: PBMCs can reveal novel gene expression changes in association with controlled dietary intervention.