Influence of dietary supplementation with dextrin or oligofructose on the hepatic redox balance in rats

Authors

  • Carina E. P. Kozmus,

    Corresponding author
    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
    • Departamento de Farmacologia e Terapêutica, Faculdade de Medicina do Porto Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal Fax: +351-225513643
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  • Eduardo Moura,

    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
    2. Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal
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  • Maria P. Serrão,

    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
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  • Helena Real,

    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
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  • João T. Guimarães,

    1. Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Porto, Portugal
    2. Department of Clinical Pathology, Hospital de São João, Porto, Portugal
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  • Paula Guedes-de-Pinho,

    1. REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal
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  • Barbara P. Duarte,

    1. Department of Clinical Analysis, Faculty of Pharmacy, University of Porto, Porto, Portugal
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  • Franklim Marques,

    1. Department of Clinical Analysis, Faculty of Pharmacy, University of Porto, Porto, Portugal
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  • Maria João Martins,

    1. Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Porto, Portugal
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  • Maria A. Vieira-Coelho

    1. Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal
    2. Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal
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Abstract

We assessed the impact of oligofructose (OFS) and dextrin (DEX) as diet supplements on hepatic redox state. Rats were fed either a 10% OFS or a 10% DEX supplemented diet for 9 wk. In the DEX diet group, the levels of hepatic protein carbonylation were decreased by 63%. Total glutathione and reduced glutathione (GSH) contents were reduced in the OFS and DEX diet groups by around 20%. DEX supplementation significantly reduced oxidized glutathione (GSSG) levels resulting in a 33% increase in the GSH/GSSG ratio. The activity of the hepatic antioxidant enzymes was not changed by either OFS or DEX supplementation. OFS supplementation caused a decrease in serum levels of triglycerides (36%), cholesterol (24%), HDL (16%) and LDL (17%). DEX supplementation only reduced triglycerides (32%) and urea (22%). Both diets increased serum levels of acetate by fivefold and propionate by twofold, but DEX diet decreased butyrate levels by 75%. Due to their different composition/structure these two dietary fibers affected metabolism in different ways. Diet supplementation with 10% DEX can potentially improve host health, by protecting the liver from protein carbonylation and by improving GSH/GSSG ratio and diet supplementation with 10% OFS can improve the lipid profile.

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