High dose of dietary resveratrol enhances insulin sensitivity in healthy rats but does not lead to metabolite concentrations effective for SIRT1 expression
Article first published online: 5 JUL 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Special Issue: Resveratrol – Current Status and Outlook
Volume 55, Issue 8, pages 1197–1206, August 2011
How to Cite
Andersen, G., Burkon, A., Sulzmaier, F. J., Walker, J. M., Leckband, G., Fuhst, R., Erbersdobler, H. F. and Somoza, V. (2011), High dose of dietary resveratrol enhances insulin sensitivity in healthy rats but does not lead to metabolite concentrations effective for SIRT1 expression. Mol. Nutr. Food Res., 55: 1197–1206. doi: 10.1002/mnfr.201100292
- Issue published online: 3 AUG 2011
- Article first published online: 5 JUL 2011
- Manuscript Accepted: 16 JUN 2011
- Manuscript Revised: 15 JUN 2011
- Manuscript Received: 30 APR 2011
- German Federal Ministry of Education and Research. Grant Number: BMBF; project no. 0312252R
- Resveratrol toxicity;
Scope:trans-Resveratrol has been shown to improve insulin sensitivity and to enhance cellular glucose uptake. Evidence from recent studies indicates that these effects depend on SIRT1-pathways.
Methods and results: Since ingestion of resveratrol leads to the presence of resveratrol and resveratrol metabolites in the body, we aimed at investigating (i) whether a daily dose of 300 mg resveratrol/kg body weight in healthy male Wistar rats for a period of 8 wk affects the selected parameters of glucose and lipid metabolism and (ii) whether the resulting plasma concentrations of resveratrol metabolites were effective in modulating SIRT1 expression. The dietary dose was based on the results from preceding toxicity studies. The results from the feeding experiment revealed plasma concentrations of resveratrol and its metabolites below 1 μmol/L and showed that fasting glucose and insulin levels were decreased by 35 and 41%, respectively, in the resveratrol group compared with controls. Insulin sensitivity was enhanced by 70%, whereas liver SIRT1 protein expression was not affected. Treatment of HepG2 cells with 10 μM resveratrol (1.49-fold) or its diglucuronides (1.21-fold) increased SIRT1 expression.
Conclusion: These results suggest that the improved insulin sensitivity after dietary administration of 300 mg resveratrol/kg body weight does not involve increased protein expression of SIRT1.