Wheat gliadins modified by deamidation are more efficient than native gliadins in inducing a Th2 response in Balb/c mice experimentally sensitized to wheat allergens
Article first published online: 7 DEC 2011
© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Special Issue: Carotenoids in Nutrition and Health - Developments and Future Trends
Volume 56, Issue 2, pages 336–344, February 2012
How to Cite
Gourbeyre, P., Denery-Papini, S., Larré, C., Gaudin, J.-C., Brossard, C. and Bodinier, M. (2012), Wheat gliadins modified by deamidation are more efficient than native gliadins in inducing a Th2 response in Balb/c mice experimentally sensitized to wheat allergens. Mol. Nutr. Food Res., 56: 336–344. doi: 10.1002/mnfr.201100353
- Issue published online: 14 MAR 2012
- Article first published online: 7 DEC 2011
- Manuscript Accepted: 15 SEP 2011
- Manuscript Revised: 29 JUL 2011
- Manuscript Received: 26 MAY 2011
- French Ministère de la Recherche et de la Technologie (MRT)
- Food allergy;
Scope: Wheat gluten proteins such as gliadins constitute major food allergens. Gluten can be modified industrially by deamidation which increases its solubility and enhances its use as a food ingredient. Sensitization to deamidated gluten has been reported to cause severe allergic reactions with anaphylaxis. The aim of this study was therefore to compare the sensitization and elicitation potentials of native (NG) and deamidated (DG) gliadins. The reactivity pattern of mice IgE was also compared with that of DG-allergic patients.
Methods and results: The ability of DG to sensitize Balb/c mice using intra-peritoneal administration with aluminium hydroxide as an adjuvant, and to elicit an allergic response after a challenge, was tested in comparison with NG. Mice sensitized with DG secreted higher levels of total IgE, IL-4, gliadin-specific IgE and IgG1 than mice sensitized with NG. By contrast, mice sensitized with NG produced higher levels of gliadin-specific IgG2a and INFγ. After a challenge, histamine levels were higher in mice sensitised with DG.
Conclusions: DG can sensitize mice much more efficiently than NG. Moreover, this mouse model of allergy to DG revealed an IgE reactivity pattern against purified gliadins which was very similar to that of DG-allergic patients.