Leucine induces myofibrillar protein accretion in cultured skeletal muscle through mTOR dependent and -independent control of myosin heavy chain mRNA levels
Version of Record online: 30 MAY 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 56, Issue 5, pages 741–752, May 2012
How to Cite
Haegens, A., Schols, A. M., van Essen, A. L., van Loon, L. J. and Langen, R. C. (2012), Leucine induces myofibrillar protein accretion in cultured skeletal muscle through mTOR dependent and -independent control of myosin heavy chain mRNA levels. Mol. Nutr. Food Res., 56: 741–752. doi: 10.1002/mnfr.201100695
- Issue online: 30 MAY 2012
- Version of Record online: 30 MAY 2012
- Manuscript Accepted: 22 DEC 2011
- Manuscript Revised: 14 DEC 2011
- Manuscript Received: 17 OCT 2011
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
|mnfr1738-sup-0001-figures1.tif||291K||Figure S1: Optimal amino acid concentrations facilitate leucine-induced myofibrillar but not total protein accretion. (A) C2C12 cells were differentiated for 5 days in 10% AA in DM or 30% AA in DM in the absence or presence of 5 nM IGF-I or 5 mM leucine. Medium and stimuli were refreshed every 48 h. Whole cell lysates were prepared. Subsequently, protein content was determined and protein levels were expressed as percentage of control. (B) C2C12 cells were differentiated for 4 days in the absence or presence of 5 mM leucine in 10% AA DM or 30% AA DM. Medium and leucine were refreshed every 48 h. Total RNA was extracted and cDNA synthesized. (B) MyHC-7 and (C) MyHC-4 gene expression levels were determined. (D) C2C12 cells were differentiated for 5 days in 10% AA in DM in the presence of 2 or 5 mM arginine. Whole cell lysates were prepared and MyHC-sl content was determined. * = Significant from respective control.|
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