β-Glucans are involved in immune-modulation of THP-1 macrophages

Authors

  • Wasaporn Chanput,

    Corresponding author
    1. Laboratory of Food Chemistry, Wageningen University and Research Centre, Wageningen, The Netherlands
    2. Food & Biobased Research, Wageningen University and Research Centre, Wageningen, The Netherlands
    3. Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok, Thailand
    • Cell Biology and Immunology Group, Wageningen University and Research Centre, Wageningen, The Netherlands
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  • Marit Reitsma,

    1. Food & Biobased Research, Wageningen University and Research Centre, Wageningen, The Netherlands
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  • Lennart Kleinjans,

    1. Food & Biobased Research, Wageningen University and Research Centre, Wageningen, The Netherlands
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  • Jurriaan J. Mes,

    1. Food & Biobased Research, Wageningen University and Research Centre, Wageningen, The Netherlands
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  • Huub F. J. Savelkoul,

    1. Cell Biology and Immunology Group, Wageningen University and Research Centre, Wageningen, The Netherlands
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  • Harry J. Wichers

    1. Cell Biology and Immunology Group, Wageningen University and Research Centre, Wageningen, The Netherlands
    2. Laboratory of Food Chemistry, Wageningen University and Research Centre, Wageningen, The Netherlands
    3. Food & Biobased Research, Wageningen University and Research Centre, Wageningen, The Netherlands
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Correspondence: Wasaporn Chanput, Food & Biobased Research, Wageningen University and Research Centre, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands

E-mail: wasaporn.chanput@wur.nl

Fax: +31 317 483 011

Abstract

Scope

We aimed to examine different immunological aspects of β-glucans derived from different food sources (oat, barley and shiitake) on phorbol myristate acetate (PMA)-differentiated THP-1 macrophages. Commercially purified barley β-glucan (commercial BG) and lentinan were included to compare β-glucans from the same origin but different degree of purity and processing.

Methods and results

Chemical composition and molecular weight distribution of β-glucan samples were determined. Inflammation-related gene expression kinetics (IL-1β, IL-8, nuclear factor kappa B [NF-κB] and IL-10) after 3, 6 and 24 h of stimulation with 100 μg/mL β-glucan were investigated. All tested β-glucans mildly upregulated the observed inflammation-related genes with differential gene expression patterns. Similar gene expression kinetics, but different fold induction values, was found for the crude β-glucan extracts and their corresponding commercial forms. Pre-incubation of THP-1 macrophages with β-glucans prior to lipopolysaccharide (LPS) exposure decreased the induction of inflammation-related genes compared to LPS treatment. No production of nitric oxide (NO) and hydrogen peroxide (H2O2) was detected in β-glucan stimulated THP-1 macrophages. Phagocytic activity was not different after stimulation by β-glucan samples.

Conclusion

Based on these in vitro analyses, it can be concluded that the analysed β-glucans have varying levels of immunomodulating properties, which are likely related to structure, molecular weight and compositional characteristic of β-glucan.

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