Antihyperuricemic and nephroprotective effects of resveratrol and its analogues in hyperuricemic mice
Article first published online: 3 AUG 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 56, Issue 9, pages 1433–1444, September 2012
How to Cite
Shi, Y.-W., Wang, C.-P., Liu, L., Liu, Y.-L., Wang, X., Hong, Y., Li, Z. and Kong, L.-D. (2012), Antihyperuricemic and nephroprotective effects of resveratrol and its analogues in hyperuricemic mice. Mol. Nutr. Food Res., 56: 1433–1444. doi: 10.1002/mnfr.201100828
- Issue published online: 3 SEP 2012
- Article first published online: 3 AUG 2012
- Manuscript Accepted: 20 JUN 2012
- Manuscript Revised: 27 MAY 2012
- Manuscript Received: 22 DEC 2011
- NSFC. Grant Numbers: 81025025, J1103512
- JSNSF. Grant Number: BK2010365
- PCSIRT. Grant Number: IRT1020
- Hypouricemic effect;
- Structure–activity relationship
Stilbenes, of which, resveratrol is a representative compound in foods and plants, possess a variety of bioactivities including antioxidation, anti-inflammation, chemoprevention, and cardioprotection. This study was conducted to evaluate the antihyperuricemic and nephroprotective effects of resveratrol and its analogues and explore the possible mechanisms. The structure–activity relationships were analyzed.
Methods and results
Potassium oxonate-induced hyperuricemic mice were dosed by gavage with eight stilbenes. Uric acid, creatinine, and blood urea nitrogen (BUN) levels in serum and urine, clearance rate of creatinine and BUN, 24-h urate excretion, and fractional excretion of uric acid, uromodulin levels in urine and kidney were determined to evaluate renal urate handling and function. Renal protein levels of organic ion transporters were detected to elucidate the possible mechanisms. Resveratrol, trans-4-hydroxystilbene, pterostilbene, polydatin, and mulberroside A were found to have antihyperuricemic activities. These compounds together with trans-2-hydroxystilbene provided nephroprotection. Trans-3,4′,5-trimethoxystilbene and cis-combretastatin A-4 had no effects.
The uricosuric and nephroprotective actions of resveratrol and its analogues were mediated by regulating renal organic ion transporters in hyperuricemic mice, supporting their beneficial effects for the prevention of hyperuricemia. The number and position, methoxylation and glycosylation of hydroxyl groups in these trans-stilbenes were required for their effects.