Both first authors and senior authors contributed equally to this work.
Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns
Article first published online: 2 APR 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 57, Issue 7, pages 1283–1290, July 2013
How to Cite
Perez-Gordo, M., Pastor-Vargas, C., Lin, J., Bardina, L., Cases, B., Ibáñez, M. D., Vivanco, F., Cuesta-Herranz, J. and Sampson, H. A. (2013), Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns. Mol. Nutr. Food Res., 57: 1283–1290. doi: 10.1002/mnfr.201200332
- Issue published online: 1 JUL 2013
- Article first published online: 2 APR 2013
- Manuscript Accepted: 6 FEB 2013
- Manuscript Revised: 24 JAN 2013
- Manuscript Received: 30 MAY 2012
- RETIC RIRAAF. Grant Number: RD07/006/0023
- Ministerio de Educacion y Ciencia. Grant Number: AGL 2004-05220
- Cod parvalbumin;
- Epitope mapping;
- Fish allergy;
- Food allergy;
- Peptide microarray
IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain.
Methods and results
Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein.
We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.