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Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns

Authors


Correspondence: Dr. Carlos Pastor Vargas, Department of Immunology, Hospital Fundación Jimenez Diaz, Avda. Reyes Católicos 2, 28040 – Madrid, Spain

E-mailcpastor@fjd.es

Fax: +34-915448246

Abstract

Scope

IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain.

Methods and results

Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein.

Conclusion

We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.

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