Vitamin D reduces the inflammatory response and restores glucose uptake in adipocytes

Authors

  • Julie Marcotorchino,

    1. INRA, UMR1260, Marseille, France
    2. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    3. Faculté de Médecine, Aix-Marseille University, Marseille, France
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  • Erwan Gouranton,

    1. INRA, UMR1260, Marseille, France
    2. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    3. Faculté de Médecine, Aix-Marseille University, Marseille, France
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  • Beatrice Romier,

    1. INRA, UMR1260, Marseille, France
    2. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    3. Faculté de Médecine, Aix-Marseille University, Marseille, France
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  • Franck Tourniaire,

    1. INRA, UMR1260, Marseille, France
    2. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    3. Faculté de Médecine, Aix-Marseille University, Marseille, France
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  • Julien Astier,

    1. INRA, UMR1260, Marseille, France
    2. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    3. Faculté de Médecine, Aix-Marseille University, Marseille, France
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  • Christiane Malezet,

    1. INRA, UMR1260, Marseille, France
    2. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    3. Faculté de Médecine, Aix-Marseille University, Marseille, France
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  • Marie-Josèphe Amiot,

    1. INRA, UMR1260, Marseille, France
    2. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    3. Faculté de Médecine, Aix-Marseille University, Marseille, France
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  • Jean-François Landrier

    Corresponding author
    1. INSERM, UMR1062, «Nutrition, Obesity and Risk of Thrombosis», Marseille, France
    2. Faculté de Médecine, Aix-Marseille University, Marseille, France
    • INRA, UMR1260, Marseille, France
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Correspondence: Dr. Jean-François Landrier; UMR INRA 1260/INSERM 1062/Aix Marseille Université, Faculté de Médecine, 27 Boulevard Jean-Moulin, 13385 Marseille Cedex 5, France

E-mail: jean-francois.landrier@univ-amu.fr

Fax: +33-491-78-2101

Abstract

Scope

Obesity is strongly associated with low-grade inflammation, notably due to an overproduction of proinflammatory markers by adipose tissue and adipocytes as well as a vitamin D deficiency. Whether these problems are interrelated has not been clearly established.

Methods and results

In the present report, decreases in the levels of inflammatory markers such as IL-6, MCP-1, and IL-1β (mRNA and protein level) in human adipocytes and in 3T3-L1 adipocytes were observed after 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) treatment. Such treatment also decreased the expression of the TNF-α-mediated proinflammatory marker in 3T3-L1 and human adipocytes. A similar effect was observed in adipocyte-macrophage co-culture systems in which 1,25-(OH)2D3 decreased proinflammatory marker expression under basal and TNF-α-stimulated conditions. The involvement of VDR and NF-κB was confirmed in these regulations. Incubation with 1,25-(OH)2D3 also resulted in the dephosphorylation of p38, which is linked to the transcriptional induction of several Dusp family members. Functional consequences of the 1,25-(OH)2D3 treatment on glucose uptake and AKT phosphorylation were observed.

Conclusion

The improvement of both proinflammatory status and glucose uptake in adipocytes under 1,25-(OH)2D3 effect suggests that low-grade inflammation could be linked to vitamin D deficiency. This observation offers new perspectives in the context of obesity and associated physiopathological disorders.

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