Vitamin D reduces the inflammatory response and restores glucose uptake in adipocytes
Article first published online: 12 OCT 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 56, Issue 12, pages 1771–1782, December 2012
How to Cite
Marcotorchino, J., Gouranton, E., Romier, B., Tourniaire, F., Astier, J., Malezet, C., Amiot, M.-J. and Landrier, J.-F. (2012), Vitamin D reduces the inflammatory response and restores glucose uptake in adipocytes. Mol. Nutr. Food Res., 56: 1771–1782. doi: 10.1002/mnfr.201200383
- Issue published online: 6 DEC 2012
- Article first published online: 12 OCT 2012
- Manuscript Accepted: 6 SEP 2012
- Manuscript Revised: 5 SEP 2012
- Manuscript Received: 19 JUN 2012
- French Ministère de l'Enseignement Supérieur et de la Recherche
- Glucose uptake;
- Vitamin D
Obesity is strongly associated with low-grade inflammation, notably due to an overproduction of proinflammatory markers by adipose tissue and adipocytes as well as a vitamin D deficiency. Whether these problems are interrelated has not been clearly established.
Methods and results
In the present report, decreases in the levels of inflammatory markers such as IL-6, MCP-1, and IL-1β (mRNA and protein level) in human adipocytes and in 3T3-L1 adipocytes were observed after 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) treatment. Such treatment also decreased the expression of the TNF-α-mediated proinflammatory marker in 3T3-L1 and human adipocytes. A similar effect was observed in adipocyte-macrophage co-culture systems in which 1,25-(OH)2D3 decreased proinflammatory marker expression under basal and TNF-α-stimulated conditions. The involvement of VDR and NF-κB was confirmed in these regulations. Incubation with 1,25-(OH)2D3 also resulted in the dephosphorylation of p38, which is linked to the transcriptional induction of several Dusp family members. Functional consequences of the 1,25-(OH)2D3 treatment on glucose uptake and AKT phosphorylation were observed.
The improvement of both proinflammatory status and glucose uptake in adipocytes under 1,25-(OH)2D3 effect suggests that low-grade inflammation could be linked to vitamin D deficiency. This observation offers new perspectives in the context of obesity and associated physiopathological disorders.