Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex

Authors

  • Keity Souza Santos,

    Corresponding author
    1. Institute for Investigation in Immunology (iii), INCT, São Paulo, SP, Brazil
    • Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of São Paulo School of Medicine, FMUSP, São Paulo, Brazil
    Search for more papers by this author
  • Gabriele Gadermaier,

    1. Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria
    Search for more papers by this author
  • Eva Vejvar,

    1. Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria
    Search for more papers by this author
  • Helen Andrade Arcuri,

    1. Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of São Paulo School of Medicine, FMUSP, São Paulo, Brazil
    2. Institute for Investigation in Immunology (iii), INCT, São Paulo, SP, Brazil
    Search for more papers by this author
  • Clovis Eduardo Galvão,

    1. Division of Allergy, Clinics Hospital of FMUSP, São Paulo, Brazil
    Search for more papers by this author
  • Ariana Campos Yang,

    1. Division of Allergy, Clinics Hospital of FMUSP, São Paulo, Brazil
    Search for more papers by this author
  • Virgínia Maria Ferreira Resende,

    1. Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of São Paulo School of Medicine, FMUSP, São Paulo, Brazil
    2. Institute for Investigation in Immunology (iii), INCT, São Paulo, SP, Brazil
    Search for more papers by this author
  • Carlo de Oliveira Martins,

    1. Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of São Paulo School of Medicine, FMUSP, São Paulo, Brazil
    2. Heart Institute (InCor), LIM-19, University of São Paulo School of Medicine, São Paulo, Brazil
    Search for more papers by this author
  • Martin Himly,

    1. Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria
    Search for more papers by this author
  • Adriano Mari,

    1. Center for Molecular Allergology, IDI-IRCCS, Rome, Italy
    Search for more papers by this author
  • Marina Liso,

    1. Center for Molecular Allergology, IDI-IRCCS, Rome, Italy
    Search for more papers by this author
  • Debora Pomponi,

    1. Center for Molecular Allergology, IDI-IRCCS, Rome, Italy
    Search for more papers by this author
  • Heimo Breiteneder,

    1. Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Search for more papers by this author
  • Stefan Wagner,

    1. Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
    Search for more papers by this author
  • Jorge Kalil,

    1. Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of São Paulo School of Medicine, FMUSP, São Paulo, Brazil
    2. Institute for Investigation in Immunology (iii), INCT, São Paulo, SP, Brazil
    3. Heart Institute (InCor), LIM-19, University of São Paulo School of Medicine, São Paulo, Brazil
    Search for more papers by this author
  • Fátima Ferreira,

    1. Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria
    Search for more papers by this author
  • Fábio Fernandes Morato Castro

    1. Laboratory of Clinical Immunology and Allergy-LIM60, Division of Clinical Immunology and Allergy, Department of Medicine, University of São Paulo School of Medicine, FMUSP, São Paulo, Brazil
    2. Institute for Investigation in Immunology (iii), INCT, São Paulo, SP, Brazil
    3. Division of Allergy, Clinics Hospital of FMUSP, São Paulo, Brazil
    Search for more papers by this author

Correspondence: Dr. Keity Souza Santos, Instituto do Coração–INCOR, Av Dr Eneas de Carvalho Aguiar, 155–9° andar–Bloco II, CEP: 05403–900 São Paulo, Brazil

E-mailkeity@usp.br

Fax: +55-11-2661-5943

Abstract

Scope

Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients.

Methods and results

The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome.

Conclusion

Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.

Ancillary