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FilenameFormatSizeDescription
mnfr1934-sup-0001-FigureS1.jpg48KFigure S1. Disorder prediction and modelling of tertiary structure. A, The disorder profile plot shows a disorder tendency over 50% for each residue in the Man e 5 primary sequence using different software predictors. B, Electrostatic potential surfaces for Man e 5, where blue indicates positive residues and red negative residues.
mnfr1934-sup-0002-FigureS2.jpg23KFigure S2. IgE cross-inhibition using sera of Brazilian and Italian allergic patients in SPHIAa microarray. Self inhibition of Hev b 5 (left), inhibition of Hev b 5 by Man e 5 (centre) and inhibition of Hev b 5 by manioc extract (Man e, right).
mnfr1934-sup-0003-FigureS3.jpg45KFigure S3. Cross-inhibition ELISA using manioc and latex extracts. A decrease of IgE-binding to manioc and latex protein extracts was tested using sera of manioc sensitized/allergic patients (n = 7) after pre-incubation with manioc (M), latex B (LB) or latex C (LC) protein extracts. Mean values and standard error of mean are shown as bar and whiskers, respectively. Statistical evaluation was performed using the Student's t-test. ns, not significant
mnfr1934-sup-0004-s1.doc54KSupporting Information

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