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Supporting Information Fig. S1. OTA down-regulates Nrf2 protein level in LLC-PK1 cells. LLC-PK1 cells were treated with 25 μM OTA for 24h and then immunofluorescent staining was performed.

Supporting Information Fig. S2. Adenoviral overexpression of Nrf2 and HO-1 does not influence cell morphology and does not cause cell death. LLC-PK1 cells were transduced with 50 MOI of AdNrf2 or AdHO-1 as wells as with AdGFP as a control vector. Increased mRNA (A) and protein (B, C) level of HO-1 and Nrf2 was observed without changes in morphology (D) and LDH release (E). A, mean of 4, E, mean of 3 experiments performed in duplicate, B - immunofluorescent staining, C - representative Western blots, D – representative photos, * p < 0.05 vs AdGFP-transduced cells

Supporting Information Fig. S3. DFO increases HIF activity and VEGF gene expression. LLC-PK1 cells were transfected with HRE-luc plasmid, and after 24h later stimulated with DFO (A). VEGF mRNA (B) and protein (C) level was assessed after 24h stimulation with 250 μM DFO by real-time PCR and ELISA, respectively. Mean of 3 experiments performed in duplicate

Supporting Information Fig. S4. OTA elevates expression of DGCR8 in LLC-PK1 cells. LLC-PK1 cells were treated for 24h with 25 μM OTA and then immunofluorescent staining was performed.

Supporting Information Fig. S5. Inhibition of miR-132 and miR-200c reduces OTA-mediated alterations in LLC-PK1 cells. LLC-PK1 cells were transfected with specific antagomirs (anti-miR-132 or anti-miR-200c) as well as with control anti-miR and after 24h were treated with 25 μM OTA for the next 24h. Inhibition of miR-132 counteracted the OTA-mediated reduction in Nrf2 protein (A). Immunofluorescent staining showed that both anti-miR-132 and anti-miR-200c increased the downregulated HO-1 protein level caused by OTA (B).

mnfr1897-sup-0001-TableS1.doc34KSupporting Information Table S1. List of primers for gene expression analysis by real-time PCR
mnfr1897-sup-0002-TableS2.doc34KSupporting Information Table S2. List of primers for miRNAs expression analysis by real-time PCR

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