Phenolic acid concentrations in plasma and urine from men consuming green or black tea and potential chemopreventive properties for colon cancer
Article first published online: 14 JAN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 57, Issue 3, pages 483–493, March 2013
How to Cite
Henning, S. M., Wang, P., Abgaryan, N., Vicinanza, R., de Oliveira, D. M., Zhang, Y., Lee, R.-P., Carpenter, C. L., Aronson, W. J. and Heber, D. (2013), Phenolic acid concentrations in plasma and urine from men consuming green or black tea and potential chemopreventive properties for colon cancer. Mol. Nutr. Food Res., 57: 483–493. doi: 10.1002/mnfr.201200646
- Issue published online: 12 MAR 2013
- Article first published online: 14 JAN 2013
- Manuscript Accepted: 21 NOV 2012
- Manuscript Revised: 8 NOV 2012
- Manuscript Received: 2 OCT 2012
- National Institutes of Health. Grant Numbers: [RO1 CA116242], [P50CA92131]
- Black tea;
- Green tea;
- HCT116 colon cancer cells;
- Phenolic metabolites
Tea polyphenols are metabolized by the colonic microflora yielding phenolic metabolites, which may contribute to the health benefits of tea. We determined the serum and urine concentrations of phenolic acids, hippuric acid, and polyhydroxyphenyl-γ-valerolactones during green tea (GT) and black tea (BT) administration. The effects of (–)-epigallocatechin gallate (EGCG) and 3,4-dihydroxyphenylacetic acid (3,4-DHPAA) alone and in combination on bioavailability, intracellular metabolism, and antiproliferative activity were determined in HCT-116 colon cancer cells.
Methods and results
The concentration of phenolic metabolites was quantified by HPLC with electrochemical detection and MS. Urine concentrations of 4-hydroxyphenylacetic acid (4-HPAA), 3-hydroxyphenylacetic acid (3-HPAA), and polyhydroxy-γ-valerolactones were increased significantly in men drinking GT compared to control. Urine concentration of 3-O-methylgallic acid (3OMGA) was significantly increased in men drinking BT compared to control. Serum 3,4-DHPAA was significantly increased after consumption of GT and BT and 4-HPAA after GT consumption. In vitro treatment of HCT-116 colon cancer cells with 3,4-DHPAA and EGCG exhibited an additive antiproliferative effect, while methylation of 3,4-DHPAA was significantly decreased. 3OMGA exhibited the strongest antiproliferative activity among the phenolic acids.
The consumption of both, GT and BT, was associated with a significant increase in urinary and serum phenolic acids.