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Figure S1. Effect of AP on epididymal (A) and subcutaneous (B) adipocyte size frequency distribution of male Wistar rats fed with HFS diet supplemented with or without AP for 56 days. Data are means ± SE (n = 4–5); * p < 0.05, ** p < 0.01 versus. control group. # p < 0.05 versus. HFS group.

Figure S2. Effect of AP on respiratory quotient (RQ; A), energy expenditure (B), activity (C) and rearing (D) of male Wistar rats fed with HFS diet supplemented with or without AP for 56 days. Data are means ± SE (n = 6); *, p < 0.05; **, p < 0.01 versus control group.

Figure S3. Effect of AP on glucose tolerance of male Wistar rats fed with HFS diet supplemented with or without AP for 56 days. Glucose curves (A) and area under the curves (B) of a 90-min IPGTT are shown. For the IPGTT 12-hr-fasted rats were acutely injected (intraperitoneally) with glucose. Data are means ± SE (n = 5); * p < 0.05, ** p < 0.01 versus control group. # p < 0.05, ## p < 0.01 versus HFS group.

Figure S4. Effect of AP on basal and isoproterenol-stimulated lipolysis of isolated epididymal adipocytes from male Wistar rats fed with HFS diet supplemented with or without AP for 56 days. Data are means ± SE (n = 5); $, p < 0.05 versus the corresponding basal values.

Figure S5. Effect of AP on mRNA levels in gastrocnemius muscle from male Wistar rats fed with HFSdiet supplemented with or without AP during 56 days. Results were normalized to 18S mRNA levels and expressed as fold change respect to controls (control set at unit). Data are means ± SE (n = 5–6); * p < 0.05 versus control group.

Figure S6. Nucleotide sequences of the CpG island in genes promoter regions showing individual CpG dinucleotides; effect of AP on the percentage of methylation of CpG dinucleotides in gene promoters of isolated epididymal adipocytes from male Wistar rats fed with HFS diet supplemented with or without AP during 56 days. (A) Aebp1 gene promoter, (B) Aqp7 gene promoter, (C) Lep gene promoter, (D) Plin gene promoter, (E) Ppargc1a gene promoter, (F) Srebf1 gene promoter. Data are means ± SE (n = 5–6); * p < 0.05, ** p < 0.01 versus control group. # p < 0.05, ## p < 0.01 versus HFS group. Grey numbers indicate CpG dinucleotides not determined due to the used technique.

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