Current address: Ionelia Taranu, Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti No. 1, Balotesti, Ilfov 077015, Romania
Deoxynivalenol impairs the immune functions of neutrophils
Article first published online: 21 FEB 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Special Issue: Cocoa and Human Health
Volume 57, Issue 6, pages 1026–1036, June 2013
How to Cite
Gauthier, T., Waché, Y., Laffitte, J., Taranu, I., Saeedikouzehkonani, N., Mori, Y. and Oswald, I. P. (2013), Deoxynivalenol impairs the immune functions of neutrophils. Mol. Nutr. Food Res., 57: 1026–1036. doi: 10.1002/mnfr.201200755
- Issue published online: 7 JUN 2013
- Article first published online: 21 FEB 2013
- Manuscript Accepted: 15 DEC 2012
- Manuscript Revised: 12 DEC 2012
- Manuscript Received: 13 NOV 2012
- French INRA-AFSSA National Coordination Program PORC-DON
- French-Japanese SAKURA project
- European Project MYCORED. Grant Number: (FP7-KBBE-2007-2A)
- Pig neutrophils
Deoxynivalenol (DON), a mycotoxin produced by Fusarium spp., is toxic to many animal species, with pigs being the most sensitive species to the toxin. The aim of the present study was to determine the effects of DON on pig polymorphonuclear cells (PMNs), the first line of defense against infection.
Methods and results
PMNs isolated from pig blood samples were stimulated with LPS to mimic infection. DON (0.5–10 μM) altered three main functions of pig PMNs: LPS-induced secretion of IL-8, chemotaxis, and phagocytosis capability. This alteration of PMN properties was due to apoptotis induced by DON exposure. Using Western blot and flow cytometry, we demonstrated that this process included the permeabilization of the mitochondrial outer membrane and the activation of caspase-3. The effect of DON was mediated by the phosphorylation of the p38 mitogen-activated protein kinase within the first 30 min of exposure.
This study provides evidence that low concentrations of DON can alter the immune functions of porcine PMNs and suggests the involvement of p38 mitogen-activated protein kinase in the signal transduction pathway. These immunosuppressive effects of DON may have implications for humans and/or animals when eating contaminated food/feed.