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Figure S1. Chemical structure of xanthohumol.

Figure S2. Decreased cholesterol concentrations in VLDL fraction of XN-fed Ldlr-/- mice. Lipoprotein profile of pooled plasma samples from overnight fasted Ldlr-/- mice (n=4) fed WTD or WTD ± XN for 4 weeks.

Figure S3. Unchanged fractional cholesterol absorption. (A) Fractional cholesterol absorption in ApoE-/- mice (n=5) fed WTD ± XN for 4 weeks. (B) Lipid levels in jejunum of ApoE-/- mice (n=9) fed WTD ± XN for 8 weeks.

Figure S4. Quantitative real-time PCR analysis of hepatic genes involved in cholesterol transport. Female ApoE-/- mice were fed WTD ± XN for 8 weeks. Livers were excised and total RNA was isolated. Data are expressed as mean values (n=4) ±SD normalized to cyclophilin A.

Table S1. Reduced plasma cholesterol concentrations in XN-fed Ldlr-/- mice.

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