These authors equally contributed to this study.
Endoplasmic reticulum stress in adipose tissue determines postprandial lipoprotein metabolism in metabolic syndrome patients
Article first published online: 12 AUG 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 57, Issue 12, pages 2166–2176, December 2013
How to Cite
Camargo, A., Meneses, M. E., Rangel-Zuñiga, O. A., Perez-Martinez, P., Marin, C., Delgado-Lista, J., Paniagua, J. A., Tinahones, F. J., Roche, H., Malagon, M. M., Perez-Jimenez, F. and Lopez-Miranda, J. (2013), Endoplasmic reticulum stress in adipose tissue determines postprandial lipoprotein metabolism in metabolic syndrome patients. Mol. Nutr. Food Res., 57: 2166–2176. doi: 10.1002/mnfr.201300036
- Issue published online: 4 DEC 2013
- Article first published online: 12 AUG 2013
- Manuscript Accepted: 4 JUN 2013
- Manuscript Revised: 27 APR 2013
- Manuscript Received: 15 JAN 2013
- European Union. Grant Number: 505944
- Ministerio de Ciencia e Innovacion. Grant Numbers: AGL2004–07907, AGL2006–01979, AGL2009–12270
- CIBER Fisiopatologia de la Obesidad y Nutricion. Grant Number: CB06/03/0047
- Consejeria de Innovacion, Ciencia y Empresa, Junta de Andalucia. Grant Number: P06-CTS-01425
- Consejeria de Salud, Junta de Andalucia. Grant Numbers: 06/128, 07/43, PI-0193
- Adipose tissue;
- Endoplasmic reticulum stress;
- Metabolic syndrome;
- Postprandial state
Our aim was to ascertain whether the quality and quantity of fat in the diet may influence the ER stress at the postprandial state in adipose tissue by analyzing the gene expression of chaperones, folding enzymes, and activators of the UPR.
Methods and results
A randomized, controlled trial conducted within the LIPGENE study assigned 39 MetS patients to one of four diets: high-SFA (HSFA; 38% energy (E) from fat, 16% E as SFA), high MUFA (HMUFA; 38% E from fat, 20% E as MUFA), and two low-fat, high-complex carbohydrate (LFHCC; 28% E from fat) diets supplemented with 1.24 g/day of long-chain n-3 PUFA or placebo for 12 wk each. A fat challenge reflecting the same fatty acid composition as the original diets was conducted post intervention. sXBP-1 is induced in the postprandial state irrespective of the diet consumed (p < 0.001). BiP increases postprandially after consumption of diets HMUFA (p = 0.006), LFHCC (p = 0.028), and LFHCC n-3 (p = 0.028). Postprandial mRNA expression levels of CRL, CNX, PDIA3, and GSTP1 in AT did not differ between the different types of diets.
Our results suggest that upregulation of the unfolded protein response at the postprandial state may represent an adaptive mechanism to counteract diet-induced stress.