Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention
Article first published online: 19 JUN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 57, Issue 11, pages 1908–1917, November 2013
How to Cite
Lim, S., Xu, J., Kim, J., Chen, T.-Y., Su, X., Standard, J., Carey, E., Griffin, J., Herndon, B., Katz, B., Tomich, J. and Wang, W. (2013), Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention. Mol. Nutr. Food Res., 57: 1908–1917. doi: 10.1002/mnfr.201300040
- Issue published online: 28 OCT 2013
- Article first published online: 19 JUN 2013
- Manuscript Accepted: 21 APR 2013
- Manuscript Revised: 18 APR 2013
- Manuscript Received: 14 JAN 2013
- USDA Cooperative. Grant Number: KS410-0214022
- graduate student summer stipend
- Terry Johnson Center for Basic Cancer Research, Kansas State University
- NIH. Grant Number: K-INBRE P20RR016475
- Colorectal cancer prevention;
- Purple-fleshed sweet potato;
- SW480 colon cancer cells
Anthocyanins, the natural pigments in plant foods, have been associated with cancer prevention. However, the content of anthocyanins in staple foods is typically low and the mechanisms by which they exert anticancer activity is not yet fully defined.
Methods and results
We selected an anthocyanin-enriched purple-fleshed sweet potato clone, P40, and investigated its potential anticancer effect in both in vitro cell culture and in vivo animal model. In addition to a high level of total phenolics and antioxidant capacity, P40 possesses a high content of anthocyanins at 7.5 mg/g dry matter. Treatment of human colonic SW480 cancer cells with P40 anthocyanin extracts at 0–40 μM of peonidin-3-glucoside equivalent resulted in a dose-dependent decrease in cell number due to cytostatic arrest of cell cycle at G1 phase but not cytotoxicity. Furthermore, dietary P40 at 10–30% significantly suppressed azoxymethane-induced formation of aberrant crypt foci in the colons of CF-1 mice in conjunction with, at least in part, a lesser proliferative PCNA and a greater apoptotic caspase-3 expression in the colon mucosal epithelial cells.
These observations, coupled with both in vitro and in vivo studies reported here, suggest anthocyanin-enriched sweet potato P40 may protect against colorectal cancer by inducing cell-cycle arrest, antiproliferative, and apoptotic mechanisms.