Effects of supplementing different ratios of omega-3 and omega-6 fatty acids in western-style diets on cow's milk protein allergy in a mouse model
Version of Record online: 19 JUN 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 57, Issue 11, pages 2029–2038, November 2013
How to Cite
Thang, C. L., Boye, J. I., Shi, H. N. and Zhao, X. (2013), Effects of supplementing different ratios of omega-3 and omega-6 fatty acids in western-style diets on cow's milk protein allergy in a mouse model. Mol. Nutr. Food Res., 57: 2029–2038. doi: 10.1002/mnfr.201300061
- Issue online: 28 OCT 2013
- Version of Record online: 19 JUN 2013
- Manuscript Accepted: 21 APR 2013
- Manuscript Revised: 30 MAR 2013
- Manuscript Received: 21 JAN 2013
- Agriculture and Agri-Food Canada
- Food allergy;
- Mouse model;
- Omega 3 fatty acids;
- Western-style diet
This study investigated the effects of supplementing different ratios of omega-6 and omega-3 fatty acids (O6H = 10:1, O3O6 = 4:1, and O3H = 1:4) to western-style diets on cow β-lactoglobulin (BLG) induced allergic reactions in Balb/c mice.
Methods and results
Three-week-old mice were randomly assigned to three diet groups (n = 20/group). At 9 wk of age, half of the mice from each dietary treatment (n = 10) were intraperitoneally (i.p.) sensitized with three weekly doses of BLG and alum while the remaining half from each group was sham sensitized (controls). One week after the final sensitization, all mice were orally challenged with BLG. Elevated BLG-specific serum Igs were observed in all sensitized and challenged mice. IFN-γ, MCP-1, and IL-12p40 concentrations from lymphocytes of mesenteric lymph nodes were highest in O3H mice, compared to O3O6 and O6H mice. O6H mice had the highest IL-4 concentrations from splenic lymphocytes and a significantly lower rectal temperature after the challenge in comparison to O3O6 and O3H mice.
Our results suggest that the ω-3 PUFA rich diets alleviated the severity of allergic reactions, and may modulate immune response toward T helper cell (Th)1-favoured immune response while the ω-6 PUFA rich diet exhibited no allergy alleviation with a stronger Th2 polarized immune response.