1,25(OH)2-vitamin D3 enhances the stimulating effect of leucine and insulin on protein synthesis rate through Akt/PKB and mTOR mediated pathways in murine C2C12 skeletal myotubes
Article first published online: 9 AUG 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 57, Issue 12, pages 2137–2146, December 2013
How to Cite
Salles, J., Chanet, A., Giraudet, C., Patrac, V., Pierre, P., Jourdan, M., Luiking, Y. C., Verlaan, S., Migné, C., Boirie, Y. and Walrand, S. (2013), 1,25(OH)2-vitamin D3 enhances the stimulating effect of leucine and insulin on protein synthesis rate through Akt/PKB and mTOR mediated pathways in murine C2C12 skeletal myotubes. Mol. Nutr. Food Res., 57: 2137–2146. doi: 10.1002/mnfr.201300074
- Issue published online: 4 DEC 2013
- Article first published online: 9 AUG 2013
- Manuscript Accepted: 28 MAY 2013
- Manuscript Revised: 22 APR 2013
- Manuscript Received: 13 FEB 2013
- Nutricia Advanced Medical Nutrition, Danone Research, Centre for Specialised Nutrition, Wageningen, the Netherlands
- National Institute for Agricultural Research
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Supporting Figure S1. Characterization of the differentiation state of C2C12 myotubes before 1,25(OH)2D3 treatment.
Supporting Figure S2. Cellular morphology of 5-day differentiated C2C12 myotubes treated with 0 or 10 nM 1,25(OH)2D3 for 72 hours.
Supporting Figure S3. Representative western blot showing the level of phosphorylated and total proteins involved in the translation initiation and elongation steps in C2C12 myotubes stimulated or not with insulin + leucine and treated or not with 10 nM 1,25(OH)2D3 .
Supporting Figure S4. Representative western blot showing the level of Insulin Receptor (IR) and Vitamin D Receptor (VDR) proteins in C2C12 myotubes treated or not with 10 nM 1,25(OH)2D3
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