Free fatty acid effects on myokine production in combination with exercise mimetics
Article first published online: 5 MAY 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Special Issue: Lipidomics: Approaches and Applications in Nutrition Research
Volume 57, Issue 8, pages 1456–1467, August 2013
How to Cite
Sánchez, J., Nozhenko, Y., Palou, A. and Rodríguez, A. M. (2013), Free fatty acid effects on myokine production in combination with exercise mimetics. Mol. Nutr. Food Res., 57: 1456–1467. doi: 10.1002/mnfr.201300126
- Issue published online: 2 AUG 2013
- Article first published online: 5 MAY 2013
- Manuscript Accepted: 27 FEB 2013
- Manuscript Received: 14 FEB 2013
- Manuscript Revised: 14 FEB 2013
- Spanish Government. Grant Number: AGL2009-11277
- European Commission. Grant Number: FP7-244995
- Fat-muscle crosstalk;
- Muscle Cells
We aimed to study the effects of free fatty acids (FFAs) alone and combined with the exercise mimetics adrenaline and 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) in the production of IL6, IL15 and Irisin in muscle cells, using a time-sequential model.
Methods and results
Differentiated C2C12 myotubes were treated with FFA, adrenaline or AICAR alone for 0, 1, 3, 8, 12 and 24 h and with double or triple combinations for 0, 3 and 24 h. Levels of mRNA in cells and protein in the medium were measured. Adrenaline, AICAR and FFA showed no significant effects on Irisin expression, while the presence in the culture of adrenaline and/or AICAR decreased IL15 mRNA expression. On contrary, the three signals showed a deep, rapid impact on the IL6 induction, especially when both AICAR and FFA were present.
The different response in IL6 versus IL15 regulation may be explained by their different energy-activating versus muscle-cell-hypertrophy suggested roles, considering that adrenaline and AMPK are involved in the activation of energy-generating pathways. Moreover, the results suggest FFAs are components that may regulate IL6 production and may have a role in muscle-adipose tissue crosstalk.