These authors contributed equally to this work.
Heat treatment of egg white controls allergic symptoms and induces oral tolerance to ovalbumin in a murine model of food allergy
Article first published online: 26 AUG 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 2, pages 394–404, February 2014
How to Cite
Watanabe, H., Toda, M., Sekido, H., Wellner, A., Fujii, T., Henle, T., Hachimura, S. and Nakajima-Adachi, H. (2014), Heat treatment of egg white controls allergic symptoms and induces oral tolerance to ovalbumin in a murine model of food allergy. Mol. Nutr. Food Res., 58: 394–404. doi: 10.1002/mnfr.201300205
- Issue published online: 3 FEB 2014
- Article first published online: 26 AUG 2013
- Manuscript Accepted: 18 JUN 2013
- Manuscript Revised: 14 JUN 2013
- Manuscript Received: 19 MAR 2013
- KIEIKAI research foundation
- Egg white;
- Food allergy;
- Heat treatment;
- Mouse model;
- Oral tolerance
Heated foods often present low allergenicity, and have recently been used in specific oral immunotherapy for food allergies. However, the influence of heating on tolerogenicity of food allergens is not well elucidated. Here, we investigated biochemical, allergenic, and tolerogenic properties of heated egg white (EW) using a murine model of food allergy.
Methods and results
Raw EWs were treated at 80°C for 15 min (80EW, mild heating condition), 100°C for 5 min (100EW, cooking condition), or 121°C for 40 min (121EW, retort pouch condition), and freeze-dried. A transgenic OVA23–3 mice model expressing T-cell receptor specific for ovalbumin (OVA, a major EW allergen) induced Th2 cells and IgE production, and presented intestinal inflammation when fed untreated EW diet. 80EW-fed mice presented only moderate inflammation but high Th2 responses. 100EW-fed mice did not present inflammation but induced tolerance as seen in reduced T-cell responses and IgE levels. 100EW demonstrated higher digestive stability and slower absorption in intestine, compared with untreated EW and 80EW. 121EW was strongly aggregated, was not absorbed well, and developed Th1 responses without tolerance induction.
OVA in EW treated only under a particular heat condition (e.g. 100°C for 5 min) lost allergenicity, but possessed tolerogenicity.