Probiotics determine hypolipidemic and antioxidant effects in hyperlipidemic hamsters
Article first published online: 18 SEP 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 3, pages 559–568, March 2014
How to Cite
Stancu, C. S., Sanda, G. M., Deleanu, M. and Sima, A. V. (2014), Probiotics determine hypolipidemic and antioxidant effects in hyperlipidemic hamsters. Mol. Nutr. Food Res., 58: 559–568. doi: 10.1002/mnfr.201300224
- Issue published online: 4 MAR 2014
- Article first published online: 18 SEP 2013
- Manuscript Accepted: 28 JUL 2013
- Manuscript Revised: 23 JUL 2013
- Manuscript Received: 27 MAR 2013
- Romanian Academy
- National Ministry of Education and Research. Grant Number: PN-II-PT-PCCA-2011–3.1–0184
- European Social Found through Sectorial Operational Program Human Resources Development 2007–2013. Grant Number: POSDRU/89/1.5/S/63258
- Oxidative stress;
Hyperlipidemia, hyperglycemia, and the oxidative stress are among the known risk factors of atherosclerosis. Our aim was to assess the hypolipidemic and antioxidant effects of a probiotic mix (Lactobacillus acidophilus and Bifidobacterium animalis) in hyperlipidemic hamsters (HL).
Methods and results
Male Golden Syrian hamsters developed hyperlipidemia after 21 weeks of fat diet. For the last 5 weeks of experiment, ten HL were treated with the probiotic mix (HLP), ten received water (HL). Ten animals received standard chow (N). Increase of plasma total cholesterol (TC), triglycerides (TG), phospholipids (PL), oxidized LDL, glucose, of 4-hydroxynonenal (4-HNE) in plasma, liver, and myocardium, and of intestinal Niemann Pick C1 like 1 (NPC1L1) and microsomal TG transfer protein (MTTP) expression was observed in HL versus N. The probiotic mix decreased plasma TC, TG, PL, oxidized LDL, 4-HNE, and glucose levels and increased paraoxonase-1 activity, decreased NPC1L1 and MTTP protein expression compared to HL. In HLP liver, a significant reduction of TC, TG, and fatty acids was observed. PL increased and 4-HNE levels decreased in the liver and myocardium of HLP versus HL.
Our data support the administration of probiotics to humans because of their hypolipidemic (through decreasing intestinal NPC1L1 and MTTP) and antioxidant effects (stimulating HDL-associated paraoxonase-1).