Postprandial fatty acid specific changes in circulating oxylipins in lean and obese men after high-fat challenge tests

Authors

  • Katrin Strassburg,

    1. Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands
    2. Netherlands Metabolomics Centre, Leiden, The Netherlands
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    • Both authors contributed equally.

  • Diederik Esser,

    1. Top Institute Food and Nutrition, Wageningen, The Netherlands
    2. Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
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    • Both authors contributed equally.

  • Rob J. Vreeken,

    1. Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands
    2. Netherlands Metabolomics Centre, Leiden, The Netherlands
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  • Thomas Hankemeier,

    1. Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands
    2. Netherlands Metabolomics Centre, Leiden, The Netherlands
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  • Michael Müller,

    1. Top Institute Food and Nutrition, Wageningen, The Netherlands
    2. Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
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  • John van Duynhoven,

    1. Netherlands Metabolomics Centre, Leiden, The Netherlands
    2. Unilever Research and Development, Vlaardingen, The Netherlands
    3. Laboratory of Biophysics, Wageningen University, Wageningen, The Netherlands
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  • Jolanda van Golde,

    1. Top Institute Food and Nutrition, Wageningen, The Netherlands
    2. Department of Internal Medicine, Maastricht University Medical Centre, Maastricht, The Netherlands
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  • Susan J. van Dijk,

    1. Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
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  • Lydia A. Afman,

    1. Top Institute Food and Nutrition, Wageningen, The Netherlands
    2. Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
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  • Doris M. Jacobs

    Corresponding author
    1. Netherlands Metabolomics Centre, Leiden, The Netherlands
    2. Unilever Research and Development, Vlaardingen, The Netherlands
    • Correspondence: Dr. Doris M. Jacobs, Unilever Research and Development, Olivier van Noortlaan 120, 3130 AC Vlaardingen, The Netherlands

      E-mail: Doris.Jacobs@unilever.com

      Fax: +31-10-460-5310

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Abstract

Scope

Circulating oxylipins may affect peripheral tissues and are assumed to play an important role in endothelial function. They are esterified in triglyceride-rich lipoproteins that are increased after a high-fat (HF) meal, depending on BMI and fatty acid (FA) type. Yet, it is unclear which oxylipins appear in circulation after HF meals differing in FA composition.

Methods and results

In a double-blind randomized crossover challenge study, we characterized the postprandial oxylipin response after different HF challenges in lean and obese men receiving HF milkshakes, either high in saturated FAs (SFA), monounsaturated FAs (MUFA), or omega 3 (n-3) polyunsaturated FAs (PUFA). Plasma oxylipin profiles were significantly altered at 2 and 4 h after shake consumption when compared to baseline. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) derived oxylipins increased after n-3 PUFA shake consumption. MUFA shake consumption increased levels of cytochrome P450 mediated oxylipins. SFA shake consumption led to strong increases in linoleic acid (LA) derived HODEs. No differences were observed between lean and obese individuals at baseline and after any shake consumption.

Conclusion

We are the first demonstrating acute effects on circulating oxylipins after HF meal challenges. These changes were strongly influenced by different dietary FAs and may affect endothelial function.

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