Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy
Article first published online: 1 OCT 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 3, pages 635–646, March 2014
How to Cite
Radosavljevic, J., Nordlund, E., Mihajlovic, L., Krstic, M., Bohn, T., Buchert, J., Velickovic, T. C. and Smit, J. (2014), Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy. Mol. Nutr. Food Res., 58: 635–646. doi: 10.1002/mnfr.201300403
- Issue published online: 4 MAR 2014
- Article first published online: 1 OCT 2013
- Manuscript Accepted: 30 JUL 2013
- Manuscript Revised: 22 JUL 2013
- Manuscript Received: 3 JUN 2013
- Ministry of Education and Science of the Republic of Serbia. Grant Number: 172024
- Protein cross-linking;
The cross-linking of proteins by enzymes to form high-molecular-weight protein, aggregates can be used to tailor the technological or physiological functionality of food products. Aggregation of dietary proteins by food processing may promote allergic sensitization, but the effects of enzymatic cross-linking of dietary proteins on the allergenic potential of food are not known. In this study, the bioavailability and the sensitizing or tolerizing potential of peanut proteins (PE) cross-linked with microbial tyrosinase from Trichoderma reesei and mushroom tyrosinase from Agaricus bisporus, were investigated.
Methods and results
The impact of cross-linking of PE on the in vitro bioavailability of fluorescein isothiocyanate-labeled peanut proteins was tested in a Caco-2 cell monolayer and by competitive ELISA. The in vivo allergenicity or capacity to induce oral tolerance in mice were measured by serum levels of PE-specific antibodies and T cell cytokine production after exposure to PE and cross-linked PE.
Enzymatic processing of peanut proteins by the two tyrosinases increased the bioavailability of major peanut allergen Ara h 2, but did not significantly change the allergenic or tolerizing properties of peanut. Enzymatic treatment of peanut proteins yielded cross-linked proteins with preserved molecular and immunological features of peanut allergens.