These authors contributed equally to this work.
Immunological behavior of in vitro digested egg-white lysozyme
Article first published online: 1 OCT 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 3, pages 614–624, March 2014
How to Cite
Jiménez-Saiz, R., Benedé, S., Miralles, B., López-Expósito, I., Molina, E. and López-Fandiño, R. (2014), Immunological behavior of in vitro digested egg-white lysozyme. Mol. Nutr. Food Res., 58: 614–624. doi: 10.1002/mnfr.201300442
- Issue published online: 4 MAR 2014
- Article first published online: 1 OCT 2013
- Manuscript Accepted: 6 AUG 2013
- Manuscript Revised: 29 JUL 2013
- Manuscript Received: 19 JUN 2013
- Egg allergy;
- Gastrointestinal digestion;
- IgE binding;
- PBMC stimulation
Besides its antimicrobial properties, lysozyme (LYS) is one of the major allergens from hen egg. This paper addresses the identification of the peptides produced upon in vitro gastrointestinal digestion of LYS, together with their IgE-binding and biological activity as a contribution to the understanding of what makes it a relevant allergen.
Methods and results
Simulated in vitro gastrointestinal digestion together with IgE binding, basophil degranulation, and peripheral blood mononuclear cells stimulation experiments were carried out. Identification of the fragments released was performed by HPLC-MS/MS and the immunoreactive products were analyzed by MALDI-TOF/TOF. Results showed that in vitro gastric and gastroduodenal digests of LYS maintained IgE binding, basophil activation capacity, and preserved T-cell immunogenicity. These biological activities could be attributed to either the persistence of intact LYS, due to incomplete gastric degradation and subsequent duodenal precipitation, the formation of fragment f(24–129) by chymotrypsin action on the soluble intact protein, or the release, upon combined gastric and pancreatic digestion, of immunoreactive peptides linked by disulphide bonds containing the epitopes f(57–83) and f(108–122).
The pH of gastric hydrolysis greatly determined the extent of subsequent duodenal digestion of LYS and the disclosure of relevant epitopes that could increase its allergenic potential.