High vitamin D and calcium intakes reduce diet-induced obesity in mice by increasing adipose tissue apoptosis
Article first published online: 22 JAN 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 6, pages 1342–1348, June 2014
How to Cite
Sergeev, I. N. and Song, Q. (2014), High vitamin D and calcium intakes reduce diet-induced obesity in mice by increasing adipose tissue apoptosis. Mol. Nutr. Food Res., 58: 1342–1348. doi: 10.1002/mnfr.201300503
- Issue published online: 5 JUN 2014
- Article first published online: 22 JAN 2014
- Manuscript Accepted: 13 DEC 2013
- Manuscript Revised: 4 NOV 2013
- Manuscript Received: 11 JUL 2013
- US Department of Agriculture (USDA). Grant Numbers: SD00H325, 2009-35200-05008
- Diet-induced obesity;
- Vitamin D
Modulation of apoptosis is emerging as a promising antiobesity strategy because removal of adipocytes through this process will result in reducing body fat. Effects of vitamin D on apoptosis are mediated via multiple signaling pathways that involve common regulators and effectors converging on cellular Ca2+. We have previously shown that 1,25-dihydroxyvitamin D3 induces the Ca2+ signal associated with activation of Ca2+-dependent apoptotic proteases in mature adipocytes. In this study, a diet-induced obesity (DIO) mouse model was used to evaluate the role of vitamin D and calcium in adiposity.
Methods and results
DIO mice fed high vitamin D3, high Ca, and high D3 plus high Ca diets demonstrated a decreased body and fat weight gain, improved markers of adiposity and vitamin D status (plasma concentrations of glucose, insulin, adiponectin, 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, parathyroid hormone (PTH)), but an increased plasma Ca2+. High D3 and Ca intakes were associated with induction of apoptosis and activation of Ca2+-dependent apoptotic proteases, calpain and caspase-12, in adipose tissue of DIO mice. The combination of D3 plus Ca was more effective than D3 or Ca alone in decreasing adiposity.
The results imply that high vitamin D and Ca intakes activate the Ca2+-mediated apoptotic pathway in adipose tissue. Targeting this pathway with vitamin D and Ca supplementation could contribute to the prevention and treatment of obesity. However, this potentially effective and affordable approach needs to be evaluated from a safety point of view.