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Effect of heat processing on antibody reactivity to allergen variants and fragments of black tiger prawn: A comprehensive allergenomic approach

Authors

  • Sandip D. Kamath,

    1. School of Pharmacy and Molecular Science, Centre for Biodiscovery and Molecular Development of Therapeutics, James Cook University, Townsville, Queensland, Australia
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  • Anas M. Abdel Rahman,

    1. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada
    2. School of Pharmacy, Yarmouk University, Irbid, Jordan
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  • Astrid Voskamp,

    1. Department of Immunology, Monash University, Melbourne, Victoria, Australia
    2. Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia
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  • Toshikazu Komoda,

    1. School of Food, Agricultural, and Environmental Sciences, Miyagi University, Sendai, Miyagi, Japan
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  • Jennifer M. Rolland,

    1. Department of Immunology, Monash University, Melbourne, Victoria, Australia
    2. Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia
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  • Robyn E. O'Hehir,

    1. Department of Immunology, Monash University, Melbourne, Victoria, Australia
    2. Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital and Monash University, Melbourne, Victoria, Australia
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  • Andreas L. Lopata

    Corresponding author
    1. School of Pharmacy and Molecular Science, Centre for Biodiscovery and Molecular Development of Therapeutics, James Cook University, Townsville, Queensland, Australia
    • Correspondence: Associate Professor Andreas L. Lopata, Molecular Immunology Group, School of Pharmacy and Molecular Science, Centre for Biodiscovery and Molecular Development of Therapeutics, James Cook University, Townsville, QLD 4811, Australia

      E-mail: andreas.lopata@jcu.edu.au

      Fax: +61-747816078

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Abstract

Scope

Prawn allergy is one of the leading causes of IgE-mediated hypersensitivity to food. Alterations of IgE-antibody reactivity to prawn allergens due to thermal processing are not fully understood. The aim of this study was to analyze the impact of heating on prawn allergens using a comprehensive allergenomic approach.

Methods and results

Proteins from raw and heat-processed black tiger prawn (Penaeus monodon) extracts as well as recombinant tropomyosin (rPen m1) were analyzed by SDS-PAGE and immunoblotting using sera from 16 shellfish allergic patients. IgE antibody binding proteins were identified by advanced mass spectroscopy, characterized by molecular structure analysis and their IgE reactivity compared among the prepared black tiger prawn extracts. Heat processing enhanced the overall patient IgE binding to prawn extracts and increased recognition of a number of allergen variants and fragments of prawn allergens. Allergens identified were tropomyosin, myosin light chain, sarcoplasmic calcium binding protein, and putative novel allergens including triose phosphate isomerase, aldolase, and titin.

Conclusion

Seven allergenic proteins are present in prawns, which are mostly heat-stable and form dimers or oligomers. Thermal treatment enhanced antibody reactivity to prawn allergens as well as fragments and should be considered in the diagnosis of prawn allergy and detection of crustacean allergens in processed food.

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