Metabolic effects of a high-fat diet post-weaning after low maternal dietary folate during pregnancy and lactation
Article first published online: 15 JAN 2014
© 2014 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Molecular Nutrition & Food Research
Volume 58, Issue 5, pages 1087–1097, May 2014
How to Cite
McKay, J. A., Xie, L., Manus, C., Langie, S. A. S., Maxwell, R. J., Ford, D. and Mathers, J. C. (2014), Metabolic effects of a high-fat diet post-weaning after low maternal dietary folate during pregnancy and lactation. Mol. Nutr. Food Res., 58: 1087–1097. doi: 10.1002/mnfr.201300615
- Issue published online: 22 APR 2014
- Article first published online: 15 JAN 2014
- Manuscript Accepted: 2 DEC 2013
- Manuscript Revised: 26 NOV 2013
- Manuscript Received: 21 AUG 2013
- BBSRC. Grant Number: BB/G007993/1
- High-fat diet;
- Maternal folate intake;
- Metabolic syndrome
Investigate the influence of low-folate supply during pregnancy and lactation on obesity and markers of the metabolic syndrome in offspring, and how provision of a high-fat diet post weaning may exacerbate the resultant phenotype.
Methods and results
Female C57Bl/6 mice were randomized to low or normal folate diets (0.4 or 2 mg folic acid/kg diet) prior to and during pregnancy and lactation. At 4 wk of age, offspring were randomized to high- or low-fat diets, weighed weekly and food intake assessed at 9 and 18 wk old. Adiposity was measured at 3 and 6 months. Plasma glucose and triacylglycerol (TAG) concentrations were measured at 6 months.
Maternal folate supply did not influence adult offspring body weight or adiposity. High-fat feeding post weaning increased body weight and adiposity at 3 and 6 months (p > 0.001). Maternal low folate lowered plasma glucose (p = 0.010) but increased plasma TAG (p = 0.048). High-fat feeding post weaning increased plasma glucose and TAG (p = 0.023, p = 0.049 respectively). Offspring from folate-depleted (but not folate-adequate) dams had 30% higher TAG concentration when fed the high-fat diet from weaning (p = 0.005 for interaction).
Inadequate maternal folate intake has long-term effects on offspring metabolism, manifested as increased circulating TAG, particularly in offspring with high-fat intake post weaning.