Diallyl sulfide as a potential dietary agent to reduce TNF-α- and histamine-induced proinflammatory responses in A7r5 cells

Authors

  • Cheng-Ying Ho,

    1. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
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  • Chia-Jui Weng,

    1. Graduate Institute of Applied Living Science, Tainan University of Technology, Yongkang Distric, Tainan City, Taiwan
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  • Jhih-Jia Jhang,

    1. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
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  • Yu-Ting Cheng,

    1. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
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  • Shang-Ming Huang,

    1. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
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  • Gow-Chin Yen

    Corresponding author
    1. Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
    2. Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan
    • Correspondence: Professor Gow-Chin Yen, Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan

      E-mail: gcyen@nchu.edu.tw

      Fax: +886-4-2285-4378

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Abstract

Scope

Oxidative stress-aggravated chronic inflammatory diseases of the airway are well documented; hence, treatment with antioxidants to ameliorate oxidative stress might be an effective strategy to reduce airway complications. The aim of this study was to investigate the effect and molecular mechanism of diallyl sulfide (DAS), which is a natural organosulfuric compound found in garlic, on the inhibition of tumor necrosis factor-alpha (TNF-α)- or histamine-induced inflammation in rat aortic smooth muscle A7r5 cells.

Methods and results

A7r5 cells were coincubated with DAS before exposure to TNF-α or histamine. DAS significantly blocked the accumulation of the nuclear p65 protein in TNF-α-induced A7r5 cells by attenuating the TNF-α receptor complex through the dissociation of the TNF receptor-associated death domain and TNF receptor-associated factor 2. Moreover, DAS inhibited histamine-induced inflammation by decreasing reactive oxygen species (ROS) levels by enhancing the nuclear factor-erythroid 2-related factor 2-related antioxidative enzyme. DAS also inhibited inflammation by suppressing interleukin-1β and TNF-α through the inhibition of ROS-induced PI3K/Akt and the downstream NF-κB and activator protein-1.

Conclusion

Our results demonstrate that DAS is a potential phytochemical to inhibit TNF-α- and histamine-induced inflammation, suggesting that DAS might be an effective dietary agent for the prevention of oxidative stress-induced inflammation of the airway.

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