Effects of obesity/fatty acids on the expression of GPR120

Authors

  • Francisca Rodriguez-Pacheco,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    2. CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Sara Garcia-Serrano,

    Corresponding author
    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    2. CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain
    • Correspondence: Dr. Eduardo Garcia-Fuentes, Unidad de Gestion Clinica de Endocrinología y Nutrición, Hospital Regional Universitario, Plaza del Hospital Civil s/n, 29009 Malaga, Spain

      E-mail: edugf1@gmail.com

      Fax: +34-95228670

      Additional correspondence: Dr. Eva Garcia-Escobar, E-mail: eyring@gmail.com

    Search for more papers by this author
    • These authors contributed equally to this work.

  • Eva Garcia-Escobar,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    2. CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain
    Search for more papers by this author
  • Carolina Gutierrez-Repiso,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    Search for more papers by this author
  • Juan Garcia-Arnes,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    Search for more papers by this author
  • Sergio Valdes,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    2. CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain
    Search for more papers by this author
  • Montserrat Gonzalo,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    Search for more papers by this author
  • Federico Soriguer,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    2. CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain
    3. CIBEROBN, Instituto de Salud Carlos III, Malaga, Spain
    Search for more papers by this author
  • Francisco J. Moreno-Ruiz,

    1. Unidad de Gestion Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    Search for more papers by this author
  • Alberto Rodriguez-Cañete,

    1. Unidad de Gestion Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    Search for more papers by this author
  • Jose L. Gallego-Perales,

    1. Unidad de Gestion Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    Search for more papers by this author
  • Abelardo Martinez-Ferriz,

    1. Unidad de Gestion Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    Search for more papers by this author
  • Gemma Rojo-Martínez,

    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    2. CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain
    3. CIBEROBN, Instituto de Salud Carlos III, Malaga, Spain
    Search for more papers by this author
  • Eduardo Garcia-Fuentes

    Corresponding author
    1. Unidad de Gestion Clinica de Endocrinogía y Nutrición, Instituto de Investigaciones Biomédicas de Málaga (IBIMA), Hospital Regional Universitario, Malaga, Spain
    2. CIBERDEM, Instituto de Salud Carlos III, Malaga, Spain
    3. CIBEROBN, Instituto de Salud Carlos III, Malaga, Spain
    • Correspondence: Dr. Eduardo Garcia-Fuentes, Unidad de Gestion Clinica de Endocrinología y Nutrición, Hospital Regional Universitario, Plaza del Hospital Civil s/n, 29009 Malaga, Spain

      E-mail: edugf1@gmail.com

      Fax: +34-95228670

      Additional correspondence: Dr. Eva Garcia-Escobar, E-mail: eyring@gmail.com

    Search for more papers by this author

Errata

This article is corrected by:

  1. Errata: Erratum Volume 59, Issue 11, 2338, Article first published online: 11 November 2015

Abstract

Scope

The effects that fatty acids (FAs) exert on G protein-coupled receptor-120 (GPR120) levels, a receptor for FAs, are still unknown. We analyzed the association between GPR120 and obesity, and the FA effects on its expression.

Methods and results

GPR120 levels were analyzed in visceral adipose tissue (VAT) from nonobese and morbidly obese subject. VAT GPR120 mRNA and protein levels were lower in morbidly obese subjects (p = 0.004). After, these subjects underwent a high-fat meal. GPR120 mRNA expression in peripheral blood mononuclear cells in the fasting state was lower in morbidly obese subjects (p = 0.04), with a decrease 3 h after a high-fat meal only in morbidly obese subjects (p = 0.043). Also, incubations of visceral adipocytes from these subjects were made with different FAs. In nonobese subjects, palmitic, oleic, linoleic, and docosahexaenoic acids produced an increase in GPR120 mRNA and protein levels (p < 0.05). In morbidly obese subjects, only linoleic acid produced an increase in GPR120 mRNA and protein levels (p < 0.05).

Conclusion

Morbidly obese subjects had lower GPR120 mRNA and protein levels in VAT and a lower mRNA expression after a high-fat meal in peripheral blood mononuclear cells. The FAs effect on GPR120 mRNA and protein levels in visceral adipocytes was lower in morbidly obese subjects.

Ancillary