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FilenameFormatSizeDescription
mnfr2151-sup-0001-Figure1.jpg149KSupplementary Figure 1. SDS-PAGE electrophoresis of protein profiles at different times during enzymatic digestion of unprocessed amaranth flour and extruded amaranth flour with pepsin during 180 min (A and B, respectively), followed by another enzymatic digestion with pancreatin (C and D, respectively) during 180 min. Total digestion was carried out during 360 min (3 h). All experiments were performed in at least three independent replicates.
mnfr2151-sup-0001-Figure2.jpg85KSupplementary Figure 2. Effect of unprocessed (A, C) and extruded (B, D) amaranth flour hydrolysates on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in LPS-induced RAW 264.7 macrophages. Different letter means statistically different (p < 0.05) relative to the positive control (C+). All treatments contain lipopolysaccharide (LPS, 1 μg/mL) except the negative control (C-) treated with PBS-alone and served as the negative control.
mnfr2151-sup-0001-Figure3.jpg133KSupplementary Figure 3. Effect on human THP-1 macrophages-like cells of amaranth hydrolysates on the secretion of TNF-α (A and B); PGE2 (E and F) and COX-2 expression (I and J) treated with unprocessed and extruded amaranth, respectively. The effect on RAW 264.7 macrophages of amaranth hydrolysates on the secretion of TNF-α (C and D); PGE2 (G and H) and COX-2 expression (K and L) treated with unprocessed and extruded amaranth, respectively. All experiments were performed in at least three independent replicates. Different letter means statistically different (p < 0.05) relative to the positive control (C+). All treatments contain lipopolysaccharide (LPS, 1 μg/mL) except the negative control (C-) treated with PBS-alone and served as the negative control.

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