Additional corresponding author: Dr. Young Ju, E-mail: firstname.lastname@example.org
Long-term exposure to dietary sources of genistein induces estrogen-independence in the human breast cancer (MCF-7) xenograft model
Article first published online: 24 FEB 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 59, Issue 3, pages 413–423, March 2015
How to Cite
Andrade, J. E., Ju, Y. H., Baker, C., Doerge, D. R. and Helferich, W. G. (2015), Long-term exposure to dietary sources of genistein induces estrogen-independence in the human breast cancer (MCF-7) xenograft model. Mol. Nutr. Food Res., 59: 413–423. doi: 10.1002/mnfr.201300780
- Issue published online: 3 MAR 2015
- Article first published online: 24 FEB 2014
- Manuscript Accepted: 20 DEC 2013
- Manuscript Revised: 18 DEC 2013
- Manuscript Received: 21 OCT 2013
- National Institute On Aging. Grant Number: P01AG024387
- National Center for Complementary and Alternative Medicines. Grant Number: P50AT006268
- National Cancer Institute
- Athymic mice;
- Breast cancer progression;
- Soy protein isolate
The long-term effect of exposure to relevant dietary levels of genistein (GEN) on estrogen receptor-positive (ER+) human breast cancer (MCF-7) progression after GEN withdrawal in athymic mice xenograft model was studied.
Materials and methods
Feeding studies were conducted to determine the estrogenic effect of diets on MCF-7 tumor growth: (1) implantation (19 weeks) and withdrawal (6 weeks) of 17β-estradiol (E2); (2) dietary GEN 500 and 750 ppm during treatment/withdrawal for 23/10 and 15/9 weeks, respectively; and, (3) dietary soy protein isolate (SPI) containing GEN 180 ppm for 31/9 weeks of treatment/withdrawal. MCF-7 tumors grew fast in the presence of E2 implantation and abruptly regressed completely after E2 withdrawal. At different rates, dietary GEN alone (500 and 750 ppm) and GEN (180 ppm)-containing SPI stimulated MCF-7 tumor growth. After removal of the stimulus diet, tumors induced by 750 ppm GEN, but not 500 ppm GEN or SPI, regressed completely. The protein expression of epidermal growth factor receptor 2 (HER2) was higher in the GEN- and SPI-induced nonregressing (GINR) tumors compared to MCF-7 and E2 controls.
Long-term consumption of low GEN doses (≤500 ppm) promotes MCF-7 tumor growth and results in GINR tumors with more aggressive and advanced growth phenotypes.