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A nondigestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid, α-glucosyl-isoquercitrin, on glucagon-like peptide 1 (GLP-1) secretion in rat intestine and enteroendocrine cells

Authors

  • Panchita Phuwamongkolwiwat,

    1. Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
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  • Tohru Hira,

    1. Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
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  • Hiroshi Hara

    Corresponding author
    1. Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
    • Correspondence: Professor Hiroshi Hara, Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060–8589, Japan

      E-mail: hara@chem.agr.hokudai.ac.jp

      Fax: +81-11-706-2504

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Abstract

This study conducted in vivo and in situ experiments with rats to investigate the glucagon-like peptide-1 (GLP-1) secretion in response to oral or ileal administration of α-glucosyl-isoquercitrin (20–40 mmol in 2 mL; Q3G), fructooligosaccharides (200 mmol in 2 mL; FOS) and Q3G + FOS. Direct effects on GLP-1-producing l-cells were also examined by an in vitro study using a murine enteroendocrine cell line, GLUTag. To evaluate the plasma GLP-1 level, blood samples from jugular cannula for in vivo and portal cannula for in situ experiments were obtained before and after administration of Q3G, FOS, or Q3G + FOS. We found tendencies for increases but transient stimulation of GLP-1 secretion by Q3G in in vivo and in situ experiments. Although FOS alone did not have any effects, Q3G + FOS enhanced and prolonged high plasma GLP-1 level in both experiments. In addition, application of Q3G on GLUTag cells stimulated GLP-1 secretion while FOS enhanced the effect of Q3G. Our results suggest that Q3G + FOS possess the potential for the management or prevention of Type 2 diabetes mellitus (T2DM) by enhancing and prolonging the GLP-1 secretion via direct stimulation of GLP-1 producing l-cell.

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