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mnfr2200-sup-0001-FigS1.doc251KFigure S1. Growth curves of mice that continuously received a high vitamin D diet (HH) or were switched to a low vitamin D diet at weaning (HL) and mice that continuously received a low vitamin D diet (LL) or were switched to a high vitamin D diet at weaning (LH). Values represent the average of 16-18 mice/group with standard deviation.
mnfr2200-sup-0002-FigS2.doc87KFigure S2. Expression profile and clustering of 114 probes that are significantly affected by vitamin D and age in colon of mice. The heat map visualizes the pattern of gene expression values (log2 transformed hybridization intensities). Each column represents one sample and each row represents one probe. There are 3 biological replicates for each of the vitamin D (low, L or high, H) and age (21 days, D21 or 3 months, 3Mo) groups. The colors indicate the degree of expression, red represents upregulated expression and green represents downregulated expression. The dendrogram at the top represents the degree of similarity between samples, based on the expression values of the 114 probes in each sample.
mnfr2200-sup-0003-FigS3.doc177KFigure S3. Fecal microbial composition of dams on a high (H) or low (L) vitamin D diet. Each dot represents an individual mouse within the dot-plot and the horizontal line indicates the mean value for each group; n = 10 mice/group. Data were obtained by qPCR as in Figures 2 and 3.
mnfr2200-sup-0001-TableS1.xlsx45KTable S1. Genes highlighted green contain a VDRE based on Wang, T. T. et al., Mol Endocrinol 2005, 19, 2685-2695.

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