Get access

Modulation of human miR-17–3p expression by methyl 3-O-methyl gallate as explanation of its in vivo protective activities

Authors

  • Valeria Curti,

    1. Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, Pavia University, Pavia, Italy
    2. Department of Earth and Environmental Sciences, Pavia University, Pavia, Italy
    Search for more papers by this author
  • Enrica Capelli,

    1. Department of Earth and Environmental Sciences, Pavia University, Pavia, Italy
    Search for more papers by this author
  • Federica Boschi,

    1. Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, Pavia University, Pavia, Italy
    Search for more papers by this author
  • Seyed Fazel Nabavi,

    1. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
    2. National Elites Foundation of Iran, Tehran, Iran
    Search for more papers by this author
  • Andria I. Bongiorno,

    1. Department of Biology and Biotechnology “L. Spallanzani,” University of Pavia, Pavia, Italy
    Search for more papers by this author
  • Solomon Habtemariam,

    1. Pharmacognosy Research Laboratories, Medway School of Science, University of Greenwich, Kent, UK
    Search for more papers by this author
  • Seyed Mohammad Nabavi,

    1. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
    2. National Elites Foundation of Iran, Tehran, Iran
    Search for more papers by this author
  • Maria Daglia

    Corresponding author
    1. Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, Pavia University, Pavia, Italy
    • Correspondence: Dr. Maria Daglia, Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy

      E-mail: maria.daglia@unipv.it

      Fax: +39-0382-422975

    Search for more papers by this author

Abstract

Scope

Methyl-3-O-methyl gallate (M3OMG) possesses in vivo antioxidant activity due to the partial restoration of the antioxidant enzymes, whose expression is altered in oxidative stress. Literature data suggest that miR-17–3p is a microRNA involved in the regulation of cellular redox status, interfering with transcription of the mRNAs responsible for the synthesis of antioxidant enzymes. To obtain deeper insight into the potential mechanism of action of M3OMG, the aim of this study was to investigate its effect on the expression levels of miR-17–3p in human cells.

Methods and results

Peripheral blood mononuclear cells and EVC-304 cells were treated with increasing subtoxic concentrations of M3OMG. The expression levels of miR-17–3p, extracted from cells and exosomes, were determined by quantitative real-time PCR. M3OMG induced a decrease in the miR-17–3p levels, and an increase in the levels of mRNA coding for the antioxidant enzymes, when compared to the control samples. Differently, in exosomes the expression levels of miR-17–3p were depended on the compound, its concentration, and the type of cell.

Conclusion

These results suggest a potential mechanism of action of M3OMG that, inducing the reduction of the levels of miR-17–3p and the increase of mRNA coding for antioxidant enzymes, allows to these latter to perform their protective effects.

Get access to the full text of this article

Ancillary