Methylation levels of the SCD1 gene promoter and LINE-1 repeat region are associated with weight change: An intervention study
Article first published online: 15 MAY 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 7, pages 1528–1536, July 2014
How to Cite
Martín-Núñez, G. M., Cabrera-Mulero, R., Rubio-Martín, E., Rojo-Martínez, G., Olveira, G., Valdés, S., Soriguer, F., Castaño, L. and Morcillo, S. (2014), Methylation levels of the SCD1 gene promoter and LINE-1 repeat region are associated with weight change: An intervention study. Mol. Nutr. Food Res., 58: 1528–1536. doi: 10.1002/mnfr.201400079
- Issue published online: 1 JUL 2014
- Article first published online: 15 MAY 2014
- Manuscript Accepted: 29 MAR 2014
- Manuscript Revised: 28 MAR 2014
- Manuscript Received: 5 FEB 2014
- Fondo de Investigación Sanitaria. Grant Numbers: PS09/02117, PI08/1592
- Consejería de Innovación. Grant Number: P09-CTS-5125
- II Ayudas SED (Sociedad Española de Diabetes) a Proyectos de Investigación en Diabetes Clínica y Básica
- CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM). Grant Number: CB07/08/0019
- Body weight;
- DNA methylation;
- Intervention study
Epigenetic processes may be affected by environmental factors. DNA methylation measured in LINE-1 elements (LINE-1, long interspersed nucleotide element-1) correlates with LINE-1 DNA methylation. Variations in stearoyl CoA desaturase (SCD) activity (a key enzyme in the fatty acid metabolism) may be involved in various processes that can lead to diseases such as obesity. We evaluated whether changes in diet after a nutritional intervention would be associated with changes in LINE-1 DNA methylation and/or specific methylation of SCD1 gene promoter.
Methods and results
Design: Prospective cohort intervention study with a control group. We recorded phenotypic, anthropometric, biochemical, and nutritional information at baseline and 1 year later. DNA methylation was quantified by pyrosequencing. LINE-1 DNA methylation and SCD1 gene promoter methylation levels were similar at the beginning of the study in both populations, whereas after a year these levels were higher in the control group (p < 0.001). In the intervention group, those subjects who lost weight showed higher levels of SCD1 gene promoter methylation after the intervention. Subjects with lower adherence to a Mediterranean diet experienced larger changes in LINE-1 methylation.
DNA methylation levels were associated with weight change and with adherence to a Mediterranean diet.