New shrimp IgE-binding proteins involved in mite-seafood cross-reactivity
Version of Record online: 30 JUN 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 58, Issue 9, pages 1915–1925, September 2014
How to Cite
Gámez, C., Zafra, M. P., Boquete, M., Sanz, V., Mazzeo, C., Ibáñez, M. D., Sánchez-García, S., Sastre, J. and del Pozo, V. (2014), New shrimp IgE-binding proteins involved in mite-seafood cross-reactivity. Mol. Nutr. Food Res., 58: 1915–1925. doi: 10.1002/mnfr.201400122
- Issue online: 8 SEP 2014
- Version of Record online: 30 JUN 2014
- Manuscript Accepted: 8 MAY 2014
- Manuscript Revised: 25 APR 2014
- Manuscript Received: 19 FEB 2014
- CIBER de Enfermedades Respiratorias
- Carlos III Institute of Health initiative
- Conchita Rábago Foundation
- Mite seafood;
Shrimp is a seafood consumed worldwide and the main cause of severe allergenic reactions to crustaceans. Seafood allergy has been related to mite sensitization, mainly mediated by tropomyosin, but other proteins could be involved. The aim of the study was to identify new shrimp allergens implicated in mite-seafood cross-reactivity (CR) in two different climate populations: dry and humid climates.
Methods and results
Shrimp and mite IgE-binding profiles of patients from continental dry and humid climates were analyzed by immunoblotting, and the most frequently recognized Solenocera melantho shrimp proteins were identified by MS as α-actinin, β-actin, fructose biphosphate aldolase, arginine kinase, sarcoplasmic calcium-binding protein, and ubiquitin. Using inhibition immunoblot assays, we demonstrate that tropomyosin and ubiquitin were responsible for mite-seafood CR from both climates; but also α-actinin and arginine kinase are implicated in dry- and humid-climate populations, respectively. Reciprocal inhibition assays demonstrated that mites are the primary sensitizer in humid-climate, as shrimp is in continental dry-climate population.
Several new shrimp allergens have been identified and should be considered in the diagnosis and treatment of shrimp allergy and mite-seafood CR. Differences in mite-seafood CR were founded to be based on the climate.